花生PDAT基因家族的全基因组生物信息学分析

Genomic Bioinformatics Analysis of PDATGene Family in Peanut

磷脂二酰甘油酰基转移酶(phospholipids:diacylglycerol acyltransferase,PDAT)催化磷脂和二酰甘油反应,生成溶血磷脂和三酰甘油,是植物三酰甘油合成的关键酶之一。为了在全基因组水平上探究花生PDAT(AhPDAT)基因家族的特征,通过生物信息学方法,结合花生基因组和转录组数据对AhPDAT家族成员从系统进化、基因结构、表达模式以及可变剪接等方面进行系统分析。结果显示,AhPDAT家族包含17个成员,不均匀分布于9条染色体上。系统进化分析发现,AhPDATs与大豆、苜蓿以及菜豆聚在一起,亲缘关系较近。基因结构分析显示,AhPDATs基因具有2~8个外显子,可编码88~742个氨基酸。表达模式分析发现,AhPDATs家族成员组织表达多样化,这种表达模式的差异表明它们功能分工的不同。同时,该家族基因还存在丰富的可变剪接类型。这些结果为今后进一步开展AhPDATs基因家族的功能研究提供参考,同时也为花生品质育种奠定了一定的理论基础。

Phospholipids:diacylglycerol acyltransferase(PDAT;EC 2.3.1.158),is one of the key enzymes in triacylglycerol(TAG)biosynthesis of plants,catalyzes phospholipids and diacylglycerols into triacylglycerols and lysophospholipids.Here,we performed bioinformatics analysis of AhPDAT gene family using genome data and transcriptome data to predict their function from aspects of gene structure,phylogenetic relationship,expression pattern and alternative splicing etc.It was shown that in peanut genome there were 17 members distributed unequally in nine chromosomes.Phylogenetic analysis showed that AhPDATs were closely related to soybean,barrel medic and string beans.Gene structure analysis showed that the exon number of these genes ranged from 2 to 8,and the protein sequence length varying from 88 to 742 amino acids.Expression pattern analysis indicated that AhPDAT genes showed diverse expression patterns,suggesting a significant divergence in their function.Meanwhile,there were rich alternative splicing types of these genes in different organs or tissues.These results will provide an important reference for functional studies of AhPDATs,and lay the foundation for quality breeding of peanut.