乌头酸基多色荧光碳点的制备及其分析应用

Fabrication of Aconitic Acid Based Carbon Dots with Multicolor Photoluminescence and Their Applications in Analysis

以乌头酸为前驱体,组合掺杂试剂乙二胺和尿素,以水热法和微波法分别制得4种光学性质优良的荧光碳点。荧光光谱显示,所制得的碳点中3种发射蓝色荧光,1种发射黄色荧光。实验选定蓝色荧光碳点中量子产率最高的AA&EDA-HT-CDs(绝对量子产率57%)和黄色荧光碳点AA&Urea-MW-CDs(绝对量子产率44%)为研究对象,对其粒径分布、元素组成以及表面基团等进行了表征。进一步研究了AA&EDAHT-CDs和AA&Urea-MW-CDs对金属离子的响应特性和细胞毒性,并将其成功用于细胞成像。

In this work,four types of water-soluble carbon dots(CDs)with excellent optical properties were fabricated through hydrothermal(HT)or microwave(MW)-assisted approach,using aconitic acid(AA)as the carbon precursor,and ethylenediamine(EDA)or urea as the co-doping reagents.Fluorescence spectroscopy showed that among these CDs,three emitted blue fluorescence and one emitted yellow fluorescence.Subsequently,one blue fluorescent AA&EDA-HT-CDs with the highest absolute quantum yield of 57%and yellow fluorescent AA&Urea-MW-CDs with an absolute quantum yield of 44%were selected,and then a series of detailed characterizations were conducted for the systematical investigation on their size distribution,chemical composition and surface groups,etc.Furthermore,the responses of as-prepared CDs toward metal ions as well as their cytotoxicity were evaluated.Finally,the prepared AA derived CDs were successfully applied in cellular bioimaging.