杂交鲟外周血细胞形态学观察

Morphological observation of peripheral blood cells in hybrid sturgeon

为了对杂交鲟(达氏鳇Huso dauricus♀×施氏鲟Acipenser schrenckii♂)外周血液中血细胞形态进行观察,采用瑞氏染色法研究其形态学特征,并依此分类。结果表明:杂交鲟血细胞由红细胞、白细胞和血栓细胞组成,其中红细胞占大多数,血栓细胞的数量仅次于红细胞,嗜酸性粒细胞较少,嗜碱性粒细胞未有发现;单核细胞体积最大,平均大小(长径×短径)为(18. 9±1. 7)μm×(16. 8±1. 7)μm,细胞核形态多样;血栓细胞的体积最小,平均大小为(8. 2±1. 2)μm×(6. 5±1. 3)μm,其着色深,一般不单独出现。本试验结果可丰富鱼类血液学研究内容,为鱼类血细胞的分类提供基础数据,并为鱼类病理及免疫等方面的研究提供基础资料。

The morphological characteristics of hemocytes in peripheral blood were investigated in hybrid sturgeon(Huso dauricus♀×Acipenser schrenckii♂)by Wright staining method.The results showed that the peripheral hematocytes of the hybrid sturgeon were composed of erythrocytes as the most abundant hematocytes,leukocytes,and thrombocytes as the secondary abundant hematocytes,with the least islandphils and without basophils.The monocytes had mean size of(18.9±1.7)μm in length×(16.8±1.7)μm in width,and the thrombocytes as the smallest blood cell with a mean size of(8.2±1.2)μm×(6.5±1.3)μm did not appear alone.The findings enriching contents of fish hematology provide the basic data for the classification of fish blood cells and offer the basic information on study of fish pathology and immunity.