红蓝光配比对桑树幼苗碳、氮代谢和内源激素的影响

Effect of different proportions of red and blue light on carbon-nitrogen metabolism and endogenous hormones in mulberry seedlings

为了探索桑树(Morus alba)幼苗生长发育对红蓝组合光的响应机制,本研究采用LEDs为光源,以桑树幼苗为研究材料,设红光(无蓝光,记为0B)、蓝光(100%B)和红蓝组合光(15%B、20%B、30%B、50%B)6个处理,以白光为对照,研究不同配比红蓝光对桑树幼苗碳氮代谢、微量元素含量、内源激素含量等方面的影响。结果表明:红光处理引起桑树幼苗碳氮代谢能力、微量元素(Mn、Cu和Zn)含量和根系活力下降,而补充蓝光却逆转或削弱了这些红光的负面效应。蓝光比例增加(0~100%)可造成桑树幼苗茎、叶中赤霉素(GA3)含量逐渐降低,红光、蓝光也可调节桑树幼苗茎对GA3的敏感性,而红蓝光配比的变化对茎叶中脱落酸(ABA)含量影响较小。由此可知,一定比例的红蓝组合光(15%B和20%B)可减弱单质红光、蓝光对桑树幼苗生理代谢的不利影响,更适于桑树幼苗生长发育。

In order to examine the response mechanisms of growth and development in mulberry seedlings to a combination of red and blue light,plants were treated with LED in this study,with six treatments using red light(0 B),blue light(100%B),and combinations of red and blue light(15%B,20%B,30%B,50%B).White light treatments were used as controls,and the effects of different proportions of red and blue light on carbon-nitrogen metabolism,microelement content,and endogenous hormone content in mulberry seedlings were studied to produce reference values for practical application of red and blue LEDs.The results showed that following red light treatments,microelements(Mn,Cu,and Zn),leaf carbon-nitrogen metabolism,and root system activity decreased,whereas supplemented blue light reversed or alleviated the adverse effects of red light.The proportional increase of blue light(0~100%)produced a gradual decrease in gibberellin(GA 3)content in the stems and leaves of mulberry seedlings,and both red light and blue light regulated the sensitivity of stems to GA 3.The proportions of red and blue light had minor effects on abscisic acid(ABA)content in stems and leaves.These results indicate that a proportionate treatment with red and blue light(15%B and 20%B)may alleviate the adverse effects of monochromatic red or blue LED light on physiological metabolism pathways of mulberry seedlings.A combined treatment with red and blue light was most favorable for growth and development of mulberry seedlings.