霍山石斛多糖吸湿保湿性能及皮肤刺激性研究

Study on Moisture-absorption-retention Capacity and Skin Irritation of Polysaccharide from Dendrobium huoshanense

为探讨霍山石斛多糖吸湿保湿性能及皮肤刺激性,应用干燥器控制湿度对霍山石斛多糖进行体外吸湿(43%、81%)和保湿性能研究,应用皮肤水分测定仪对霍山石斛多糖进行人体皮肤保湿性能研究,应用家兔正常皮肤和破损皮肤进行霍山石斛多糖皮肤刺激性研究。结果显示:霍山石斛多糖体外吸湿率随环境湿度的升高而升高,相对湿度43%、81%的吸湿率强弱为丙三醇>霍山石斛多糖>壳聚糖>海藻酸钠;体外的保湿强弱为壳聚糖>霍山石斛多糖>海藻酸钠>丙三醇,霍山石斛多糖在涂抹后6、8、12 h的人体皮肤保湿率显著高于丙三醇保湿率,12 h显著高于海藻酸钠保湿率,与壳聚糖保湿率无显著性差异;家兔正常皮肤及破损皮肤刺激值小于0. 5。表明霍山石斛多糖具有一定的吸湿保湿性能,且无皮肤刺激性,具有开发为天然保湿剂的潜能。

In order to explore moisture-absorption retention capacity and skin irritation of polysaccharide from Dendrobium hu-oshanense,the properties of moisture-absorption(43%,81%)retention of polysaccharide from Dendrobium huoshanense are studied in vitro by controlling the relative humidities of the disiccator,and studied in vivo by skin moisture meter,then the skin irri-tation of polysaccharide from Dendrobium huoshanense studied by using normal and damaged skin of rabbits.The results discover that moisture-absorption(43%、81%)of polysaccharide from Dendrobium huoshanense increase with the increase of environmental humidity,the sequence of the moisture absorption rate with the relative humidity of 43%and 81%is as follows:Glycerol>Dendrobium huoshanense polysaccharide>chitosan>sodium alginate;the sequence of in vitro moisture content is as below:chitosan>Dendrobium huoshanense polysaccharide>sodium alginate>glycerol.After smearing Dendrobium huoshanense poly-saccharide for 6 h,8 h and 12 h,the moisture retention rate of human skin is significantly higher than that of glycerol,the moisture retention rate of human skin for 12 h is significantly higher than that of sodium alginate and is not significantly different from that of chitosan;the stimulus value of normal skin and damaged skin of rabbits is less than 0.5,indicating that Dendrobium huoshanense polysaccharide has a certain moisture absorption and moisturizing performance but no skin irritation,and they boast the potential of being developed into natural moisturizer.