斑茅内生真菌Nigrospora sp.BM-2次级代谢产物研究

The Secondary Metabolites from Endophytic Fungus Nigrospora sp. BM-2 of Saccharum arundinaceum Retz

内生菌具有丰富的生物多样性,是潜力巨大的微生物新资源。内生菌的研究对于保护野生与濒危药用植物、拓展药用资源、新药研发等均有一定意义。从药用植物斑茅(Saccharum arundinaceum Retz.)的叶片中分离得到内生真菌Nigrospora sp. BM2,对其代谢产物化学成分进行研究,通过固体发酵,乙酸乙酯萃取得浸膏,其浸膏经硅胶柱层析,Sephadex LH-20,半制备型HPLC等分离手段分离得到10个化合物,经波谱学方法鉴定其结构分别为Pestalotiopyrones G(1)、Nigrosporapyrone D(2)、Pestalotiopyrones A(3)、Hydroxypestalopyrone(4)、Mellein(5)、6-hydroxymellein(6)、4-hydroxymellein(7)、5,8-dihydroxy-4-methyl-coumarin(8)、~1H-indole-3-carboxaldehyde(9)和Isosclerone(10)。体外细胞毒活性显示以上化合物对HepG2和Caski细胞均无明显抑制活性,其IC_(50)>100μM。除化合物1和5外,其余8个化合物均为首次从该属菌株的代谢产物中分离得到。

Endophytes had plentiful biological diversity,and become an potential microorganism resource.The research on endophytes could have some significance for the protection of wild and endangered medicinal plants,expanding medicinal resources and the research and development of new drugs.Chemical constituents of fermentation broth of endophytic fungus Nigrospora sp.BM-2 was isolated from the leaf of Saccharum arundinaceum Retz were studied using column chromatography,semi-preparative HPLC,ODS column chromatography and Sephadex LH-20 column chromatography,respectively.Ten compounds were obtained.Based on NMR and MS analyses,their structures were elucidated as Pestalotiopyrones G(1),Nigrosporapyrone D(2),Pestalotiopyrones A(3),Hydroxypestalopyrone(4),Mellein(5),6-hydroxymellein(6),4-hydroxymellein(7),5,8-dihydroxy-4-methyl-coumarin(8),1H-indole-3-carboxaldehyde(9)and Isosclerone(10).Preliminary in vitro cytotoxic screening showed that all the compounds has no significant inhibitory activity against HepG2 and Caski cell lines,IC 50>100μM.In addition,compounds 2-4 and 6-10 were isolated from Nigrospora sp.for the first time.