超声辅助法提取柿子叶中总黄酮工艺的研究

Ultrasound-assisted Extraction Process of Total Flavonoids from Persimmon Leaves

对柿子叶中的总黄酮利用超声波技术进行提取研究。研究5种因素条件对提取结果的影响趋势,并采用正交试验优化提取工艺。结果如下:当超声浸提次数2次、超声浸提时间50 min、超声浸提温度60℃、溶剂中乙醇浓度60%、料液比1∶27(g/mL),效果最佳,在此基础上测定提取物的得率为4.82%。

The ultrasound-assisted extraction technology of total flavonoids from persimmon leaves were studied.The effect of extraction of five factors were studied,and orthogonal test was made and optimized of extraction process was obtained.The results showed that,the optimal condition was when extraction was made for 2 times,ultrasonic extraction time was 50 min,extraction temperature was 60℃,ethanol concentration in the solvent was 60%,and solid-liquid ratio was 1∶27(g/mL).On this condition the extraction rate could be 4.82%.