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鮟鱇鱼骨胶原多肽螯合钙的工艺优化 被引量:6

Process Optimization of Calcium Chelate Collagen Peptide Chelate of Lophius litulon Bone
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摘要 为减少水产品加工过程中副产物对环境的污染与资源的浪费、提高水产品的附加值,对鮟鱇鱼骨制备胶原多肽螯合钙的工艺进行优化。以鮟鱇鱼骨为原料,在适宜条件下制备小分子胶原多肽,并与鱼骨钙提取液进行螯合,制成胶原多肽螯合钙,分别考察螯合温度、鱼骨胶原多肽与鱼骨浸提液的质量比、时间、pH值对螯合率和螯合物中钙含量的影响。根据单因素试验结果,对胶原多肽螯合钙工艺进行四因素三水平正交试验,得到鮟鱇鱼骨胶原多肽螯合钙的最佳工艺参数为螯合温度30℃、时间50 min、鮟鱇鱼鱼骨胶原多肽与钙浸提液质量比2∶1、pH 6,此条件下的螯合率和螯合物中的钙含量分别为98.00%和21.29%。对螯合钙进行氨基酸组成成分分析,其中,具备典型的胶原多肽氨基酸的含量占总氨基酸含量的17.37%。 In order to reduce the environmental pollution and waste of by-products during the process of aquatic products processing and to increase the added value of aquatic products,the process of preparation collagen chelate calcium chelate of Lophius litulon bone was studied.Collagen peptide chelated calcium was prepared under the suitable conditions with the fish bones as raw materials,and the effects of temperature,mass ratio of fish collagen peptide to fish bone extract,time,pH on chelating rate and calcium content in chelate impact.According to the results of single factor test,the collagen peptide chelating calcium process was carried out three leves orthogonal experiment of four factors,and the optimum technological parameters of calcium chelation for collagen peptide were obtained at 30℃for 50 min.The ratio of calcium chelate to Ca2+and Ca2+in the chelating solution was 2∶1 and pH 6,respectively,and the content of calcium in the chelate was 98.00%and 21.29%,respectively.The chelated calcium amino acid composition analysis,in which,with a typical collagen polypeptide amino acid content of 17.37%of the total amino acid content.
作者 何云 汪有先 赵淑静 包建强 HE Yun;WANG You-xian;ZHAO Shu-jing;BAO Jian-qiang(College of Food Science,Shanghai Ocean University,Shanghai 201306,China)
出处 《食品研究与开发》 CAS 北大核心 2018年第21期133-138,共6页 Food Research and Development
基金 水产动物遗传育种中心上海市协同创新中心(ZF1206) 上海市科委工程中心建设(11DZ2280300)
关键词 鮟鱇鱼骨 胶原多肽 螯合钙 氨基酸分析 Lophius litulon bone collagen peptides chelated calcium amino acid analysis
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