DNA条形码在厦门湾鱼卵和仔稚鱼分类鉴定中的应用

Identification of ichthyoplankton using DNA barcoding in Xiamen Bay

本研究选取2017年4月采集的厦门湾鱼卵、仔稚鱼样品进行DNA条形码分析。获得鱼卵、仔稚鱼的COI基因序列27条,共鉴定种类11种,全部鉴定到种,隶属于4目9科11属。在系统进化树的分析中,同一种类的不同个体都聚在一支,所有物种都分别聚为独立的一支,这些物种都能有效地区分开来,说明利用COI基因可以进行有效的仔稚鱼物种鉴定。同时获取11种鱼类浮游生物高清图像,并描述其甄别性的形态特征。以上结果表明,线粒体COI基因作为DNA条形码可以实现鱼卵和仔稚鱼的物种鉴定,且较多能鉴定到种的水平。

In most studies,the ichthyoplankton have been identified by morphological characteristics.Previous studies have shown that DNA barcoding can be used to identify the early life stages of fish more accurately than traditional morphological methods.To study the applicability of this technique to identify ichthyoplankton,samples were collected in Xiamen Bay in April 2017.A total of 27 valid cytochrome C oxidase subunit I(COI)gene sequences of ichthyoplankton were obtained.Eleven species were identified to the level of species.All sequences formed species units in a neighbor-joining phylogenetic tree[based on Kimura-2-parameter(K2P)distances],indicating that the DNA barcode could be used to identify these 11 species.Consequently,COI barcoding was one of the useful tools to identify ichthyoplankton.The results demonstrated that DNA barcoding was a powerful complement to traditional morphology,and an accurate,standardized,and efficient tool for ichthyoplankton species identification,and possessed a broad prospect of application in fish biodiversity research.