续断饮片标准汤中川续断皂苷Ⅵ转移率的研究

Study of Transfer Rate of Standard Decoction of Dipsacus asper Decoction Pieces

目的:研究续断饮片标准汤中川续断皂苷Ⅵ的转移率。方法:采用高效液相色谱法测定续断饮片及其标准汤中川续断皂苷Ⅵ的含量,色谱柱为SinoChrom ODS-AP,流动相为乙腈-水(30∶70,V/V),流速为1.0 mL/min,检测波长为212 nm,柱温为30℃,进样量为10μL。根据成分含量测定结果计算从饮片到标准汤转变过程中川续断皂苷Ⅵ的转移率。结果:川续断皂苷Ⅵ检测进样量线性范围为0.484～4.84μg(r=0.999 9);精密度、稳定性、重复性试验的RSD均小于2%;定量限、检测限分别为0.3、0.1μg;饮片和标准汤中的加样回收率分别为95.13%～100.22%(RSD=1.78%,n=6)、97.07%～100.08%(RSD=0.98%,n=6);耐用性试验的RSD均小于1%。续断饮片标准汤中川续断皂苷Ⅵ的转移率为26.3%～49.5%。结论:该方法操作简便、准确,精密度、稳定性、重复性、耐用性好,可用于续断饮片标准汤中川续断皂苷Ⅵ转移率的测定。

OBJECTIVE:To study transfer rate of asperosaponinⅥin standard decoction of Dipsacus asper decoction pieces.METHODS:The content of asperosaponinⅥin Dipsacus asper decoction pieces and its standard decoction was determined by HPLC.The determination was performed on SinoChrom ODS-AP with mobile phase consisted of acetonitrile-water(30∶70,V/V)at the flow rate of 1.0 mL/min.The detection wavelength was set at 212 nm,and column temperature was 30℃.The sample size was 10μL.By the ingredients content obtained,the transfer rate of asperosaponinⅥwas calculated during decoction piece to standard decoction.RESULTS:The linear range of asperosaponinⅥwas 0.484-4.84μg(r=0.999 9).RSDs of precision,stability and reproducibility tests were all lower than 2%.The limits of quantification and detection were 0.3 and 0.1μg,respectively.The average recoveries in D.asper decoction pieces and standard decoction were 95.13%-100.22%(RSD=1.78%,n=6),97.07%-100.08%(RSD=0.98%,n=6).RSD of durability test was lower than 1%.The transfer rate of asperosaponinⅥin standard decoction of D.asper decoction pieces ranged 26.3%-49.5%.CONCLUSIONS:The method is simple,accurate,precise,stable,reproducible and durable,and can be used for transfer rate of asperosaponinⅥin standard decoction of D.asper decoction pieces.