HPLC法同时测定三味龙胆花片中马钱苷酸和异金雀花素的含量

Simultaneous Determination of Loganic Acid and Isoscoparin in Sanwei Longdanhua Tablets by HPLC

目的:建立同时测定三味龙胆花片中马钱苷酸、异金雀花素含量的方法。方法:采用高效液相色谱法。色谱柱为Inertsil ODS-3,流动相为甲醇-0.2%磷酸溶液(梯度洗脱),流速为1.0 mL/min,检测波长为240 nm,柱温为30℃,进样量为10μL。结果:马钱苷酸和异金雀花素进样量线性范围分别为0.040 08～4.008 0μg(r=0.999 9)、0.021 96～2.196 0μg(r=0.999 9);定量限分别为0.160 32、0.087 8 ng/mL,检测限分别为0.080 16、0.043 92 ng/mL;精密度、稳定性、重复性试验的RSD均小于2%;加样回收率分别为103.07%～104.26%(RSD=0.52%,n=6)、95.57%～99.61%(RSD=1.55%,n=6)。结论:该方法简便、精确,可用于同时测定三味龙胆花片中马钱苷酸和异金雀花素的含量。

OBJECTIVE:To establish a method for the simultaneous determination of loganic acid and isoscoparin in Sanwei longdanhua tablets.METHODS:HPLC method was adopted.The determination was performed on Inertsil ODS-3 column with mobile phase consisted of methanol-0.2%phosphoric acid(gradient elution)at the flow rate of 1.0 mL/min.The detection wavelength was set at 240 nm,and column temperature was 30℃.The sample size was 10μL.RESULTS:The linear range were 0.040 08-4.008 0μg(r=0.999 9)for loganic acid and 0.021 96-2.196 0μg(r=0.999 9)for isoscoparin.The quantitative limits were 0.160 32 and 0.087 8 ng/mL,and detection limits were 0.080 16 and 0.043 92 ng/mL.RSDs of precision,stability and reproducibility tests were all lower than 2%.The recoveries were 103.07%-104.26%(RSD=0.52%,n=6)and 95.57%-99.61%(RSD=1.55%,n=6).CONCLUSIONS:The method is simple,accurate and suitable for simultaneous determination of loganic acid and isoscoparin in Sanwei longdanhua tablets.