长链非编码RNA BC200促进食管鳞癌侵袭与转移

Long non-coding RNA BC200 can promote the invasion and metastasis of esophageal squamous carcinoma

目的研究长链非编码RNA BC200(LncRNA BC200,BC200)可否促进食管鳞癌细胞的侵袭与转移。方法应用q RT-PCR检测食管鳞癌细胞BC200的表达。应用划痕试验和Transwell检测抑制BC200表达后食管鳞癌细胞的迁移和侵袭能力的改变。应用MTT试验检测抑制BC200表达后食管鳞癌细胞增殖和凋亡的变化。结果高侵袭细胞系KYSE150中BC200的表达水平高于配对的低侵袭细胞系KYSE450,差异有统计学意义(t=4.125,P<0.01)。高侵袭细胞系KYSE410中BC200的表达水平高于配对的低侵袭细胞系KYSE510,差异有统计学意义(t=4.539,P<0.01)。高侵袭细胞系KYSE70中BC200的表达水平高于配对的低侵袭细胞系9706,差异有统计学意义(t=5.195,P<0.01)。稳定转染BC200 sh RNA的KYSE410细胞(KD组)在划痕4 h迁移率低于阴性病毒感染的KYSE410细胞(NC组),但差异无统计学意义(t=0.397,P>0.05); KD组在划痕8 h迁移率低于NC组,差异有统计学意义(t=2.586,P<0.05)。KD组培养24 h转移细胞变化倍数低于NC组,差异有统计学意义(t=4.298,P<0.05)。KD组存活细胞比例与对照组无明显变化,差异无统计学意义(P>0.05)。结论 BC200可促进食管鳞癌细胞侵袭与转移,抑制BC200表达后食管鳞癌细胞侵袭转移能力降低,增殖和凋亡能力无变化。

Objective To verify whether the long non-coding RNA(LncRNA BC200,BC200)can promote the invasion and metastasis of esophageal carcinoma cell lines.Methods The BC200 expression of esophageal squamous carcinoma cell lines was detected by qRT-PCR.Transwell and scratch test were applied to detect the changes of invasion and metastasis of esophageal squamous carcinoma cell lines after inhibition BC200.The changes of proliferation and apoptosis were detected by MTT test after inhibition BC200.Results The BC200 level in high invasion cell line KYSE150 was higher than that of the paired low invasion cell line KYSE450,and the difference was statistically significant(t=4.125,P<0.01).The BC200 level in high invasion cell line KYSE410 was higher than that of the paired low invasion cell line KYSE510,and the difference was statistically significant(t=4.539,P<0.01).The BC200 level in high invasion cell line KYSE150 was higher than that of the paired low invasion cell line KYSE450,and the difference was statistically significant(t=5.195,P<0.01).The migration rate of KYSE410 cells transfected with BC200 shRNA(KD group)was lower than that of KYSE410 cells negative control group(NC group)at 4 hour,but the difference was no statistically significant(t=0.397,P>0.05).The migration rate of KD group was significantly lower than that of NC group at 8 hour,and the difference was statistically significant(t=2.586,P<0.05).The invasion fold in KD group was inferior to NC group at 24 hour,and the difference was statistically significant(t=4.298,P<0.05).Between KD group and NC group,surviving fraction had no obvious difference,and there was no statistically significant difference(P>0.05).Conclusion BC200 can promote invasion and metastasis of esophageal squamous carcinoma.With the knockdown of BC200,its ability of invasion and metastasis is decreased,and its ability of proliferation and apoptosis is no susceptible.