摩拉水牛二酰甘油酰基转移酶2基因的多态性检测

SNP Detection of DGAT2 Gene in Murrah Buffalo

本研究旨在检测水牛二酰甘油酰基转移酶2(diacylglycerolacylt-ransferase,DGAT2)基因的单核苷酸多态性(SNP),探究摩拉水牛多态性位点的群体遗传特征。以广西水牛研究所的57头摩拉水牛为材料,PCR扩增DGAT2基因的部分序列(外显子2及内含子2、3),通过常规测序法检测其SNP,并运用遗传多样性分析软件(POPGENE)和SPSS软件对群体的多态性位点进行基因频率、基因型频率、多态信息含量(PIC)、有效等位基因数(Ne)及遗传杂合度(He)的检测。结果表明,在摩拉水牛DGAT2基因外显子2和内含子2、3上共发现了9个SNPs位点(IVS2.54G>A、IVS2.158A>G、EVS2.191A>G、EVS2.228A>G、IVS3.311C>T、IVS3.444A>G、IVS3.451A>C、IVS3.466C>T、IVS3.521C>T),其中EVS2.191A>G位点的突变导致氨基酸由异亮氨酸突变为缬氨酸,突变位点间存在一定程度的连锁遗传但接近连锁平衡状态。从基因频率上看,IVS2.158A>G、EVS2.191A>G、IVS3.311C>T、IVS3.451A>C、IVS3.466C>T和IVS3.521C>T 6个SNPs位点的两个等位基因频率有较大差异,提示等位基因频率较大的基因个体可能更适合生存。9个SNPs位点在摩拉水牛品种上多处于高度多态,杂合度在0.1744~0.4975之间,说明摩拉水牛群体中DGAT2基因遗传多态性丰富,具有较大的育种价值和性状改良潜力。

This study was aimed to investigate the single nucleotide polymorphisms(SNP)of DGAT 2 gene in buffalo,and explore the population genetic characteristics of the polymorphism in Murrah buffalo.A total of 57 Murrah buffaloes from Guangxi buffalo research institute were selected as materials,the exon 2,introns 2 and 3 sequences of DGAT 2 gene were amplified by PCR,SNP was detected using conventional sequencing,gene frequency and genotype frequency,polymorphism information content(PIC),effective number of alleles(Ne)and detection of genetic heterozygosity(He)were analyzed by genetic diversity analysis software(POPGENE)and SPSS softwares.The results showed that 9 SNPs loci were found of DGAT 2 gene exon 2,introns 2 and 3 in Murrah buffalo(IVS2.54 G>A,IVS2.158 A>G,EVS2.191 A>G,EVS2.228 A>G,IVS3.311 C>T,IVS3.444 A>G,IVS3.451 A>C,IVS3.466 C>T and IVS3.521 C>T),EVS2.191 A>G caused the amino acid to be changed from isoleucine to valine,and there was a certain degree of linkage inheritance between the mutation sites but close to the chain equilibrium state.There was more difference between two allele frequency of 6 SNPs loci(IVS2.158 A>G,EVS2.191 A>G,IVS3.311 C>T,IVS3.451 A>C,IVS3.466 C>T and IVS3.521 C>T).It was suggested that the higher allele frequency individuals might be more suitable for survival.9 SNPs loci in DGAT 2 gene in the buffalo species were high polymorphism,the heterozygosity were 0.1744 to 0.4975,which illustrated the buffalo populations DGAT 2 gene genetic polymorphism was rich,and had a great breeding value and strain improvement potential.