水稻离体叶片纹枯病接种样品菌量检测方法研究

Studies on quantification method of rice sheath blight pathogen( Rhizoctonia solani) in inoculated rice detached-leaf samples

利用q PCR方法对水稻离体叶片纹枯病接种材料进行纹枯病菌含量的检测,从而对水稻发病程度进行定量分析。各水稻品种接种样品的纹枯病菌DNA含量,从低到高的顺序为:YSBR1、徐稻3号、台北309、泰粳394和Lemont。通过对接种实验条件的精确控制,离体叶片的病斑扫描,以及相对病斑面积的统计分析,验证了关于病害严重程度的q PCR检测结果。大田接种试验中,YSBR1、Lemont和泰粳394的平均病级呈显著差异;这进一步证明,通过纹枯病菌DNA的q PCR方法,能够快速有效地定量检测不同水稻品种的纹枯病抗性差异。该研究可为量化纹枯病发病程度、评价水稻品种的抗性水平及进行纹枯病的早期预报,提供有效的检测手段。

In this study,qPCR assays of the rice sheath blight(SB)pathogen inoculated to detached rice leaf tissues were performed to evaluate the disease level.Rice cultivars with the relative content of pathogen DNA in inoculated samples from the lowest to the highest are as below:YSBR1,Xudao 3,Taipei 309,Taijing 394,Lemont.The disease severity of leaf tissues indicated by the qPCR results was verified by precise control of the experimental conditions in SB inoculation,digital scanning of disease lesions,and statistical analysis of lesion areas.The average disease indexes of YSBR1,Lemont and Taijing 394 were significantly different from each other in field SB inoculation,further demonstrating that qPCR can be used to quantitatively evaluate the SB resistance level of rice cultivars.This study provides an effective method for quantification of the SB severity,evaluation of the resistance level of rice cultivars,and the forecast of the SB disease.