真菌诱导子对茶条槭细胞没食子酸积累的影响

Effect of Fungal Elicitor on Gallic Acid Accumulation in the Cells of Acer ginnala Maxim.

茶条槭(Acer ginnala Maxim.)叶片中含有没食子酸,但含量较低。真菌诱导子可以增加植物中一些次生代谢产物的含量,但其机理尚不十分清楚。本研究在茶条槭细胞悬浮培养的对数期加入内生真菌(Phomopsis sp.)诱导子,茶条槭细胞中没食子酸含量在24 h后开始增加,48 h时没食子酸含量达到峰值,最高含量为12. 2mg·g^(-1)DW,是对照的1. 58倍。茶条槭细胞对内生真菌诱导子的防御反应不同于对病原和非生物胁迫。真菌诱导子不提高培养液中p H值,也不明显增加胞内Ca^(2+)浓度,但增大细胞膜通透性。培养液电导率差异显著,细胞核发生分裂,说明真菌诱导子可能促进茶条槭细胞核内有丝分裂,促使茶条槭细胞对培养液中的无机盐离子的吸收,以满足细胞生长的需要。PAL酶活性升高,在48 h时为对照的1. 75倍,说明PAL酶可能参与了真菌诱导没食子酸的合成。

The leaf of Acer ginnala Maxim.contains gallic aicd,however,in a low content.The fungal elicitors increase some secondary metabolites in plant.However,the mechanism of the increase effect are not well studied.A fungal elicitor prepared with Phomopsis sp.was added during the logarithmic growth phase of suspension cultures of A.ginnala cells.As a result,the accumulation of gallic acid increased after 24 h and reached the peak(12.2 mg·g-1 DW,1.58-fold of control)at 48 h.Defensive responses of the cell to the endophytical elicitor were different from those to a pathogen or an abiotic stress.The endophytical elicitor did not induce medium alkalinization and an internal influx of calcium ions,but increased the permeability of plant plasma membrane.The medium conductivity increased and the nuclei division was observed,which indicate that the fungal elicitor promotes mitosis of the plant cells,which consumes more mineral nutrients to grow.Compared to the control,the activity of phenylalaninammo-nialyase(PAL)increased up to 1.75 times,indicating the accumulation of GA can be attributed to the rise of PAL activity.