1-氯-3-丁烯-2-醇对人胚肝L02细胞中内源性H_2S的抑制效应

Inhibition of endogenous H_2S concentrations in human hepatocyte L02 cells by 1-chloro-2-hydroxy-3-butene

1-氯-3-丁烯-2-醇(1-chloro-2-hydroxy-3-butene, CHB)是环境污染物1,3-丁二烯的一种代谢产物,具有细胞毒性、遗传毒性和致突变性. H_2S是继NO和CO之后发现的第3种气体第二信使分子,涉及多种生理功能,其代谢异常与很多疾病存在密切联系.研究了CHB对人胚肝L02细胞中内源性H_2S浓度的影响.结果发现, CHB可明显降低细胞内源性H_2S的浓度,且降低程度取决于CHB浓度的高低.因此, CHB对内源性H_2S产生的抑制效应可能是其毒性的一种机理.

1-chloro-2-hydroxy-3-butene(CHB)is a metabolite of 1,3-butadiene,an environmental pollutant.CHB is cytotoxic,genotoxic,and mutagenic.H2S is the third gasotransmitter following NO and CO,and is involved in a variety of physiological activities.Abnormal metabolism of H2S is associated with many diseases.In the present study,the effect of CHB on the concentrations of endogenous H2S in human hepatocyte L02 cells was investigated.It was observed that CHB could cause significant decreases in the concentrations of endogenous H2S in the cells and the effect depended on CHB concentration.Thus,inhibition of endogenous H2S production in cells by CHB may be a mechanism for toxicity of CHB,which can contribute to a better understanding of toxicity of 1,3-butadiene.