杜仲提取液对脂多糖诱导的乳腺炎小鼠疗效观察和作用途径

Efficacy of Du Zhong injection on lipopolysaccharide-induced mastitis in mice

目的研究杜仲提取液对小鼠乳腺炎的治疗效果和作用途径。方法通过乳导管灌注LPS构建小鼠乳腺炎模型,注射LPS 12 h后通过腹腔进行不同浓度梯度杜仲给药,分为6组。给药12 h后对小鼠进行剖杀并取其乳腺组织,利用HE染色观察各组病理变化,利用ELISA方法检测乳腺组织中TNF-2α、IL-6、IL-1β细胞因子的变化情况。结果 1)浓度为50μL 0.2 mg/mL的LPS可成功构建小鼠乳腺炎模型;(2)HE染色结果表明杜仲提取液可减轻LPS诱导的小鼠乳腺炎的损伤程度,随着注射杜仲提取液剂量的增加,乳腺组织的损伤程度减轻,且注射剂量为20 mg/kg和27 mg/kg时效果最佳;(3)ELISA结果表明杜仲提取液可降低乳腺组织中炎性细胞因子IL-1β、IL-6、TNF-α的含量,且注射剂量为27 mg/kg时效果最佳(P<0.01)。结论杜仲提取液可显著减轻小鼠乳腺组织病理损伤、减少乳腺组织内炎性细胞因子IL-1β、IL-6、TNF-α的释放,阻断了中性粒细胞向乳腺组织和乳腺腺泡腔募集和迁移过程,从而减轻乳腺组织损伤,其中浓度为27 mg/kg的杜仲提取液抗炎效果最佳。

Objective In this study,we tested the therapeutic efficacy and mechanisms of action of Du Zhong injection in a mouse mastitis model.Methods The mastitis mouse model was produced by injection of lipopolysaccharide(LPS)into the breast duct.At 12 h post LPS injection,mice were split into six groups and administered different concentrations of Eucommia ulmoides(Du Zhong)into the abdominal cavity.After a further 12 h recovery,mice were killed and the mammary glands collected.Pathological changes in each group were observed using hematoxylin and eosin staining.Changes in tumour necrosis factor-α(TNF-α),interleukin-6(IL-6)and interleukin-1(IL-1)cytokines in breast tissue were detected by enzyme-linked immunosorbent assay.Results The mouse mastitis model was successfully constructed using 50μL of 0.2 mg/ml LPS.Pathological examination revealed that,increasing doses of Du Zhong injection were associated with decreased mammary gland injury in the model mice,with the greatest effect observed at 20 mg/kg and 27 mg/kg.By enzyme-linked immunosorbent assay,Du Zhong injection also reduced pro-inflammatory cytokine expression in the breast tissue,with greatest effects observed at 27 mg/kg(P<0.01).Conclusions Du Zhong injection can significantly reduce pathological damages,pro-inflammatory cytokine release,and neutrophil invasion in mammary gland tissue in a mouse model of mastitis,with an optimal dosing of 27 mg/kg.