组合行为学实验方法检测东莨菪碱制作小鼠谵妄模型

Combined behavioral test to detect delirium induced by scopolamine in mice

目的用组合行为学实验方法检测东莨菪碱制作小鼠谵妄模型。方法比较两组小鼠行为学变化:谵妄组(n=12)和对照组(n=8)小鼠分别腹腔注射15 mg/kg东莨菪碱和等容量的生理盐水,在注射后30～60 min之间对两组小鼠顺序进行黑白箱实验10 min,旷场实验10 min和非选择性非持续性注意力实验10 min—组合行为实验法,比较两组小鼠的5项行为学指标以检测谵妄状态。结果与对照组相比,谵妄组小鼠5项行为学指标差异均有显著性,小鼠在白箱中停留的时间显著缩短,P<0.05;小鼠的活动速度显著增快,P<0.05,在沿壁区活动时间显著增多,P<0.05,僵住时间显著减少,P<0.05;小鼠"注意力"水平显著下降,P<0.05。结论组合行为实验法能反映谵妄诊断要素,更好地反映谵妄状态,且用时较短。

Objective To develop a combined behavioral test for assessment of scopolamine-induced delirium in mice.Methods Two groups of mice were randomly assigned to receive 15 mg/kg scopolamine(delirium group;n=12)or isovolemic saline(control group;n=8)via intraperitoneal injection.We developed a novel behavioral experiment in which mice received the light and black box test for 10 min,the open-field test for 10 min,and then the non-selective non-sustained attention test for 10 min,from 30 to 60 min after intervention.Five behavioral indexes were compared between the groups to assess the delirium status.Results Compared with the control group,there were significant differences in all five behavioral indexes in mice in the delirium group mice at 30 min after intervention:time spent in the white box was significantly shorter(P<0.05),the movement speeds were significantly increased(P<0.05),time spent beside the wall area was increased significantly(P<0.05),the freezing time,and the attention level were significantly decreased(P<0.05).Conclusions This combined behavioral experiment can be used to assess the key elements for delirium diagnosis,and with a faster time than conventional tests.