开设药理学非成瘾性镇痛药实验教学项目的探索

The practice and exploration of non addictive analgesic teaching experiment in pharmacology

目的探索适合课堂教学实验的非成瘾性镇痛药,延续药理学实验课经典镇痛药物药效学观察实验。方法筛选ICR小鼠100只,随机分成五组:对照组(Control)、美洛昔康组(Meloxicam)、阿司匹林组(Asipirin)、安痛定组(Antodine)、哌替啶组(Pethidine);分别采用热板法和扭体法制备小鼠疼痛模型,与对照组及成瘾性镇痛药哌替啶比较,检测各非成瘾性镇痛药的作用效果。结果扭体法显示,与对照组比较,美洛昔康组、阿司匹林组、安痛定组、哌替啶组均有显著镇痛作用(P<0.001),且阿司匹林组、安痛定组、哌替啶组优于美洛昔康组(P<0.001),安痛定组优于阿司匹林与哌替啶组(P<0.05)。热板法显示:与对照组比较,阿司匹林组、安痛定组、哌替啶组具有显著镇痛作用(P<0.05,P<0.01),而安痛定组与哌替啶组效果优于阿司匹林组(P<0.01)。结论综合以上结果表明美洛昔康、阿司匹林与安痛定作为非成瘾性镇痛剂,可以完全替代成瘾性镇痛剂进行教学实验。

Objective The possibility of using non addictive analgesics in a pharmacological teaching experiment was explored in order to continue the traditional analgesic experiment.Methods One hundred ICR mice were randomly divided into five groups:saline control group,meloxicam(5 mg/kg)group,aspirin(0.6 g/kg)group,antodine(1.2 mL/kg)group,and pethidine(5 mg/kg)group.Mouse pain models were established using hot plate and acetic acid torsional method.Each group was administered their respective substances by intraperitoneal injection.The analgesic effects were observed for the non addictive analgesics compared with those in the control group and the addictive analgesic group.Results The result showed that the analgesic effects of meloxicam,aspirin,antodine,and pethidine were significantly higher than in the control group,when tested with the acetic acid torsional method(P<0.001);those in the aspirin,antodine,and pethidine groups were superior to that in the meloxicam group(P<0.01);and that in the antodine group was superior to those in the aspirin and pethidine groups(P<0.05).Moreover,when tested with the hot plate method,the analgesic effects of aspirin,antodine,and pethidine were significant compared with that in the control group(P<0.05,P<0.01),while those in the antodine and pethidine groups were better than for aspirin(P<0.01).Conclusions As nonaddictive analgesics,meloxicam,aspirin,and antodine can take the place of addictive analgesics in teaching experiments.