ERS标志物PDI表达对肝癌细胞迁移的影响

Effects of ERS marker PDI expression on liver cancer cell migration

目的:研究内质网应激(ERS)标志物蛋白质二硫键异构酶(PDI)表达对肝癌细胞迁移的影响。方法:取人正常肝细胞株LO2、肝癌细胞株HepG2和SMMC-7721,采用无水乙醇处理48 h诱导发生ERS;采用Western blotting检测葡萄糖调节蛋白78(GRP78)、生长停滞及DNA损伤诱导基因153(CHOP)、X-盒-结合蛋白-1(XBP-1)及PDI的表达水平;观察PDI过表达对肝癌细胞迁移能力的影响。结果:Western blotting结果显示,与醇处理前比较,处理后的细胞GRP78及XBP-1表达水平升高(P <0. 05); LO2和SMMC-7721中CHOP含量显著上调(P <0. 05),但HepG2均无CHOP表达。在处理48 h后,3种细胞中PDI的表达水平均下调,表达水平从高到低为SMMC-7721、LO2、HepG2,与处理前相比较,差异有统计学意义(P <0. 05)。PDI过表达后,LO2的迁移能力降低,HepG2和SMMC-7721的迁移能力增强(P <0. 05)。结论:无水乙醇诱导ERS后,PDI在SMMC-7721及HepG2中表达水平下调; PDI表达水平与上述两种肝癌细胞迁移能力相关,即PDI过表达可促进HepG2与SMMC-7721迁移。

Objective:To study the effects of endoplasmic reticulum stress(ERS)marker protein disulfide isomerase(PDI)expression on liver cancer cell migration.Methods:The human normal liver cell line LO2,liver cancer cell line HepG2 and high transfer capability hepatocellular carcinoma cell line SMMC-7721 were taken,anhydrous ethanol was used to induce ERS for 48 h.Western blotting was used to detect ERS marker glucose regulation protein 78(GRP78),stunted growth and DNA damage induced by gene 153(CHOP),X-box-binding protein 1(XBP-1)and the expression level of PDI.The effect of PDI overexpression on the migration ability of HCC cells was observed.Results:Western blotting results showed that compared with prior to anhydrous ethanol treatment,the expression of GRP78 and XBP-1 levels were rised(P<0.05)after treatment;CHOP content in SMMC-7721 and LO2 cells was significantly raised(P<0.05),But HepG2 was not expressed in CHOP before and after anhydrous ethanol processing.After anhydrous ethanol processing for 48 h,PDI expression of three cells was down,the sequencel of PDI in expression from high to low was SMMC-7721,LO2,HepG2.Compared with that before anhydrous ethanol processing,the difference was statistically significant(P<0.05).After PDI overexpression,LO2 cell migration ability was reduced,HepG2 and SMMC-7721 cell migration ability were enhanced(P<0.05).Conclusion:After ERS induced by ethanol,PDI expression level in SMMC-7721 and HepG2 cells are cut.PDI expression level is associated with the above two kinds of liver cancer cell migration ability.The PDI expression can promote HepG2 and SMMC-7721 migration.