骨化三醇对桥本甲状腺炎大鼠血清炎性细胞因子表达的影响及其意义

Effects of calcitriol on serum inflammatory cytokines in Hashimoto's thyroiditis rats and its significance

目的:观察骨化三醇对桥本甲状腺炎大鼠血清炎性细胞因子表达的影响,探讨其临床意义。方法:将36只大鼠随机分为对照组、模型组和骨化三醇组,每组12只。模型组及骨化三醇组利用高碘诱发制备大鼠甲状腺炎模型,骨化三醇组大鼠给予骨化三醇治疗。观察各组大鼠甲状腺组织形态学变化,采用酶联免疫吸附测定(ELISA)法测定血清甲状腺球蛋白抗体(TGAb)、抗甲状腺过氧化物酶自身抗体(TPOAb)、肿瘤坏死因子-ɑ(TNF-ɑ)、白细胞介素-1(IL^(-1))、白细胞介素-6(IL-6)水平;采用化学发光法检测血清游离三碘甲状腺原氨酸(FT3)、游离甲状腺素(FT4)、促甲状腺激素(TSH)水平。结果:对照组大鼠甲状腺滤泡呈椭圆形或圆形,甲状腺上皮细胞排列均匀、整齐,未见浆细胞和淋巴细胞浸润;模型组大鼠甲状腺滤泡排列杂乱,内有浆细胞浸润,甲状腺上皮细胞排列紊乱,见明显淋巴细胞浸润;骨化三醇组甲状腺滤泡排列基本规则,偶有浆细胞浸润,甲状腺上皮细胞排列尚可,少量淋巴细胞浸润。模型组和骨化三醇组TGAb、TPOAb、FT3、FT4均高于对照组(P <0. 05),TSH均低于对照组(P <0. 05);骨化三醇组TGAb、TPOAb低于模型组(P <0. 05),骨化三醇组FT3、FT4、TSH和模型组比较差异无统计学意义(P> 0. 05)。模型组和骨化三醇组血清TNF-ɑ、IL^(-1)、IL-6水平均高于对照组(P <0. 05),骨化三醇组血清TNF-ɑ、IL^(-1)、IL-6水平低于模型组(P <0. 05)。结论:骨化三醇可通过降低血清TNF-ɑ、IL^(-1)、IL-6等细胞因子水平发挥对桥本甲状腺炎大鼠的治疗作用。

Objective:To observe the effect of calcitriol on serum inflammatory cytokines in Hashimoto s thyroiditis rats and to explore its clinical significance.Methods:36 rats were randomly divided into control group,model group and calcitriol group,each group had 12 rats.Rats in model group and calcitriol group were made thyroiditis model by high iodine.Rats in calcitriol group were treated with calcitriol.The morphological changes of thyroid tissue were observed in each group.Thyroglobulin antibody(TGAb),anti-thyroid peroxidase autoantibody(TPOAb),tumor necrosis factor-α(TNF-α),interleukin-1(IL-1)and interleukin-6(IL-6)were measured by enzyme-linked immunosorbent assay(ELISA).The levels of free triiodothyronine(FT3),free thyroxine(FT4)and thyroid stimulating hormone(TSH)were measured by chemiluminescence method.Results:In the control group,thyroid follicles were oval or round,the thyroid epithelial cells arranged evenly and neat,had no plasma cells and lymphocyte infiltration.In the model group,the thyroid follicle was arrangement disorder,and had plasma cell infiltration,thyroid epithelial cells arranged disorder,had significant lymphocyte infiltration.In the calcitriol group,thyroid follicle arrangement was basic rules,had occasional plasma cell infiltration,thyroid epithelial cells can be arranged,had a small amount of lymphocyte infiltration.The levels of TGAb,TPOAb,FT3 and FT4 in the model group and the calcitriol group were higher than those in the control group(P<0.05),the levels of TSH were lower than that in the control group(P<0.05).The levels of TGAb and TPOAb in the calcitriol group were lower than those in the model group(P<0.05).There was no significant difference in FT3,FT4,TSH between the calcitriolol group and the model group(P>0.05).The levels of serum TNF-α,IL-1 and IL-6 in the model group and the calcitriol group were higher than those in the control group(P<0.05).The levels of serum TNF-α,IL-1 and IL-6 in the calcitriol group were lower than those in the model group(P<0.05).Conclusion:Calcitriol can exert therapeutic effect on Hashimoto s thyroiditis rats by lowering serum levels of TNF-ɑ,IL-1 and IL-6.