一测多评法用于忍冬藤多成分含量测定的适用性研究

Applicability Study on QAMS for Multi-component Content Determination of Lonicerae Japonicae Caulis

目的建立一测多评法同时测定忍冬藤中绿原酸、咖啡酸、隐绿原酸、异绿原酸A、异绿原酸C、马钱苷6种成分含量的方法,并验证该法在忍冬藤质量控制中应用的可行性与适用性。方法以绿原酸为内参物,采用Waters Symmetry C18色谱柱(4.6 mm×250 mm,5μm),流动相为乙腈-0.4%磷酸,梯度洗脱,流速为1 m L/min,绿原酸、咖啡酸、隐绿原酸、异绿原酸A、异绿原酸C检测波长为327 nm,马钱苷检测波长为236 nm,柱温35℃。结果建立了忍冬藤中绿原酸与咖啡酸、隐绿原酸、异绿原酸A、异绿原酸C、马钱苷的相对校正因子;一测多评法的计算值与外标法实测值无明显差异。结论一测多评的质量控制模式可用于忍冬藤中6种不同成分的多指标同步质量评价。

Objective To establish a QAMS method for content determination of six compositions(chlorogenic acid,caffeic acid,cryptochlorogenin acid,isochlorogenic acid A,isochlorogenic acid C and loganin)from Lonicerae Japonicae Caulis;To verify the feasibility and applicability of this method in quality control of Lonicerae Japonicae Caulis.Methods Chlorogenic acid was set as internal reference substance.The HPLC analysis was performed on a Waters Symmetry C18 column(4.6 mm×250 mm,5μm)with a mobile phase consisted of acetonitrile and 0.4%phosphoric acid solution in gradient elution manner at a flow rate of 1 mL/min.The column temperature was maintained at 35℃,and the detection wavelength was set at 327 nm for chlorogenic acid,caffeic acid,cryptochlorogenin acid,isochlorogenic acid A,isochlorogenic acid C and 236 nm for loganin.Results The relative correction factors of caffeic acid,cryptochlorogenin acid,isochlorogenic acid A,isochlorogenic acid C and loganin were established;there was no obvious difference between calculated value of QAMS and measured value of external standard method.Conclusion The quality control mode of QAMS can be used for multi-index synchronization quality evaluation of the six compositions from Lonicerae Japonicae Caulis.