基于广州市政垃圾渗滤液的MFC性能及阳极微生物分析

MFC Power Output Performance and Anode Community Analysis Based on Leachate of Guangzhou Landfill

为了分离纯化可适应渗滤液极端环境的产电菌,以广州市白云区李坑和兴丰两处垃圾填埋场获取的渗滤液为底物运行微生物燃料电池(microbial fuel cell, MFC),待稳定输出多个周期后剪取阳极碳布进行单菌落培养和电镜扫描。结果显示,各组渗滤液底物MFC均能成功启动。李坑四季样的MFC峰值电压分别为0.334、0.331、0.321、0.328 V;兴丰四季样的MFC峰值电压分别为0.512、0.54、0.523、0.536 V。对各组渗滤液底物微生物燃料电池的阳极进行菌株分离纯化并单菌落培养构建阳极微生物系统发育树,发现经过MFC驯化后的阳极菌株具有较高丰度和差异性;SEM扫描发现各组实验中菌株均吸附在阳极碳布上形成稳定的膜结构,根据产电呼吸的基本电子传递机制推测渗滤液底物MFC中的微生物通过与阳极直接接触来传递电子。

To obtain exoelectrogen that can adapt extreme condition of leachate,microbial fuel cells were initiated with leachate as substrate.The leachates were collected from municipal landfill refuses which located in Likeng and Xingfeng,Baiyun district,Guangzhou.After the output was stable for several cycles,the anode carbon cloth was separated for single colony cultivation and scanning electron microscopy analysis.The results revealed that MFCs with different leachate were successfully initiated.The MFCs with Likeng samples achieved a maximum output of 0.334,0.331,0.321 and 0.328 V,respectively.In the meanwhile,the MFCs with Xingfeng samples achieved a maximum output of 0.512,0.54,0.523 and 0.536 V,respectively.Based on the bacteria separated from carbon cloth biofilm,the anode phylogenetic tree was constructed.It was found that the acclimatized strains have a relative high abundance and difference.The SEM images illuminated that the microorganism was attached to the anode surface to form biofilm,which can be speculated that the electron transfer mechanism in leachate based MFC was directly contact with the electrode.