甘薯引进种SSR遗传多样性分析

Genetic diversity analysis of introduced sweetpotato germplasm collections

通过SSR分子标记,对112份甘薯引进种进行了遗传多样性和群体结构分析,并与中国地方种进行了聚类比较分析。研究了中国目前保存最多的甘薯引进种间的遗传多样性和亲缘关系,以及与地方种间的遗传多样性关系。用SSR建立研究材料的0,1数据库,用ntsys-pc 2.10软件计算遗传距离矩阵并导入MEGA 6.06聚类,利用STRUCTURE 2.3.4软件对112份引进种进行群体结构分析,并将112份引进种与58份中国地方种共170份进行聚类分析。结果表明:112份材料的平均遗传距离为0. 552,遗传距离分布在0. 031到0. 949范围之内。使用Neighbor-Joining (NJ)聚类法,在遗传距离为0. 442处将112份材料分成9个类群,其中类群9又在遗传距离为0. 428处,分为3个亚群。亚群ⅨC含有39份材料,该亚群主要来自南美洲,聚类集中,血缘较为单一。通过群体结构分析将112份材料划分成了2个群体,通过对引进种的来源地比较分析,发现来自南美洲与其他地区种质材料分属于2个群体。通过对引进种和地方种共计170份甘薯资源聚类比较分析,发现中国大陆地方种与中国台湾品种间亲缘关系最近,与东南亚、东北亚、南美洲甘薯引进种间亲缘关系较近,与国际马铃薯中心、国际热带农业研究所的材料和世界蔬菜中心的材料亲缘关系较远。

To understand the genetic relationship among introduced sweetpotato germplasm collections and landraces sweetpotato,genetic diversity and population structure of 112 introduced sweetpotato germplasm collections were analyzed with SSR markers.The 0,1 database of the test materials was established by SSR markers.The ntsys-pc 2.10 software was used to calculate the genetic distance matrix.The genetic distance matrix was imported into MEGA 6.06 to output cluster analysis.The population structure of 112 introduced species was analyzed using STRUCTURE 2.3.4 software,and the duster analysis was performed on 12 introduced species and 58 landraces.The results showed that the average genetic distance of 112 accessions was 0.552,and the genetic distance ranged from 0.031 to 0.949.Neighbor-Joining(NJ)cluster analysis could divide 112 accessions into nine groups at 0.442 genetic distance.Among them,the ninth group was divided into three subgroups at 0.428 genetic distance.SubgroupⅨC contained 39 materials,and the main source of this subgroup was South America.The clustering of these sweetpotato germplasm collections was concentrated and the blood was comparatively simple.By analysis of population structure,the 112 materials were divided into two groups.Based on the comparative analysis of the origin of introduced species,it was found that germplasm originating from South America and other regions belonged to two groups.Through the cluster analysis of 170 sweetpotato germplasm resources,it was found that the genetic relationship between sweetpotato landraces and sweetpotato in Taiwan was closest,and the genetic relationship betweent landraces and sweetpotato introduced from Southeast Asia,Northeast Asia and South America was closer,but the landraces were for from the stweetpotato introduced from International Potato Center,World Vegetable Center.