腐皮镰孢霉菌侵染及保鲜剂处理对秋葵相关抗性酶的影响

Effects of Fusarium solani infection and preservative treatment on resistance associated enzyme from okra

为研究秋葵果实响应外界胁迫反应及合理利用果实抗性酶的保护功能,以川秋葵为试验材料,采用人工接种腐皮链孢霉菌和保鲜剂处理的方法,研究秋葵果实抗性酶的变化。结果表明,POD、PPO、PAL、C4H、4CL、CAD和TAL参与了秋葵果实响应腐皮链孢霉菌侵染和成熟衰老的反应过程。4CL、POD、PPO在整个腐皮链孢霉菌侵染阶段活性持续增高起着重要的防御作用; PAL、CAD在腐皮链孢霉菌侵染的4 d内活性增高,且在整个侵染阶段都保持了较高的活性; C4H在腐皮链孢霉菌侵染的3 d内起着重要的防御作用; TAL在侵染24 h迅速启动响应反应。不同保鲜剂对抗性酶活性表达的诱导作用不同,1-MCP与壳聚糖复合处理对维持果实抗病性和采后品质的效果最好。

In order to study the response of okra fruits to the external stress and the protection of fruit resistant enzyme,the changes of resistant enzymes in okra fruits were studied by artificially inoculating Fusarium solani and preservative treatment methods.The results showed that POD,PPO,PAL,C4H,4CL,CAD and TAL were involved in the response of okra fruits to the ripening and senescence and infection of Fusarium solani.4CL,POD and PPO played an important role in the defense of the constantly increasing activity during the infection stage of Fusarium solani.The activities of PAL and CAD increased within four days of the inoculation of Fusarium solani and maintained the relatively high activity throughout the infection stage.C4H played an important defense role within three days of the infection by Fusarium solani.The response of TAL was activated rapidly within 24 hours of infection.However,different preservatives had different induction effects on the active expression of resistant enzyme.The combination of 1-MCP and chitosan was best for maintaining the disease resistance of fruits and postharvest quality.