基于SLAF-seq技术的烟草抗CMV主效QTL定位

CMV Resistance QTL Analysis in Tobacco Based on SALF-seq Technique

烟草花叶病(CMV)是烟草主要病害之一,筛选与CMV抗病QTL紧密连锁的分子标记是烟草抗病分子育种的重要部分。本研究以抗病材料台烟8号和感病材料NC82为亲本,获得BC1群体,构建抗、感池。采用BSA和SLAF-seq技术开发CMV抗病相关SNP分子标记,共获得180 370个SLAF标签,经分析后获得6156个多态性SNP标记。利用SNP-index分析方法,发现两个与CMV抗病高度关联的区域。利用这些分子标记成功定位到了两个CMV抗病相关QTL,其中一个QTL定位于Chr01上的55.72～60.44 Mb区间内,区间大小为4.72 Mb;另一个则定位于烟草Chr18上的44.21～48.70 Mb区间内,区间大小为4.49 Mb。在关联到的QTL区间内设计分子标记可以用于烟草抗CMV育种的辅助选择。

Cucumber mosaic virus(CMV)is one of most important diseases in tobacco,and selection of molecular markers closely linked to CMV resistant QTLs is a key for tobacco CMV-resistance breeding.In order to identify genes closely linked to CMV resistance and develop resistant varieties,a BC1 population derived from resistant-parent Taiyan-8 and susceptible-parent NC82 was used to construct resistant and susceptible pools.By application of BSA and SLAF-seq technique,a total of 180,370 SALF labels were obtained and 6,156 polymorphic SNP markers were developed.As a result,two CMV-associated regions were identified by analysis of SNP-index data.Our results showed that abundant polymorphic SNPs can be developed between tobacco varieties using SALF-seq.By utilization of these SNPs,two CMV-resistance QTLs were successfully identified in Chr01 and Chr18,with 4.72 Mb and 4.49 Mb interval,respectively.The two identified QTLs are useful for in molecular marker assisted selection in tobacco breeding programs for CMV resistance.