烟草赤星病菌的Biolog代谢表型分析

Biolog Analysis of Metabolic Phenotypes of Tobacco Brown Spot Pathogens

为了解我国烟草赤星病的2种主要致病菌链格孢菌Alternaria alternata和长柄链格孢菌Alternaria longipes的碳氮源代谢表型。采用Biolog表型芯片技术分析了2株链格孢菌(中等致病力的CQ1和GZ11)及2株长柄链格孢菌(强致病力的HuN2和弱致病力的YN4)对PM1、PM2微孔板中190种碳源物质和PM3微孔板中95种氮源物质的代谢情况。结果发现,4株赤星病菌均能代谢PM1-PM3微孔板中24.21%的碳源和86.31%的氮源。不同致病力菌株间的碳氮源代谢能力表现出一定差异,长柄链格孢菌的强致病力菌株HuN2比弱致病力菌株YN4对D-甘露糖、D-木糖、D-甘露醇、b-环糊精、L-缬氨酸、D-天门冬酰胺、腺苷和i-赤藓糖醇等物质的代谢更显著;链格孢菌的中等致病力菌株CQ1比GZ11对L-鼠李糖、海带多糖和二羟基丙酮的代谢更好;弱致病力菌株YN4比强致病力菌株HuN2和中等致病力菌株CQ1、GZ11对水杨苷的代谢更明显。表明烟草赤星病菌的不同种群菌株对碳氮源的代谢情况总体趋势一致,但不同致病力菌株间存在一定差异。

Tobacco brown spot is a fungal disease occurring at the late stage of tobacco maturation,which is mainly harmful to tobacco leaf and seriously affects tobacco production in China.In order to understand the carbon and nitrogen source utilization phenotypes of Alternaria alternata and Alternaria longipes which are two main pathogenic fungi of tobacco in China.CQ1(moderately virulent)and GZ11(moderately virulent)of A.alternata as well as HuN2(most virulent)and YN4(weak virulent)of A.longipes were selected to analyze the utilization of 190 species of carbon source materials in the microplates of PM1 and PM2,95 species of nitrogen source of PM3 using the Phenotype Microarray System.The results revealed that the utilization of carbon and nitrogen sources of the four tested strains were roughly similar,the utilization ratio of carbon and nitrogen sources were 24.21%and 86.31%,respectively.There were also some differences among them,for A.longipes,the most virulent HuN2 had a better use of D-Mannose,D-Xylose,D-Mannitol,b-Cyclodextrin,L-Valine,D-Asparagine,L-Asparagin,Adenosine,i-Erythritol,and so on than the weak virulent YN4.For A.alternata,the moderately virulent CQ1 had a better ability in using L-Rhamnose,Laminarin and Dihydroxyacetone than GZ11.Additionally,the weak pathogenic strain YN4 was better than HuN2,CQ1 and GZ11 in using Salicin which indicated that there were obvious utilization variance in different types of pathogenicity.