肝癌组织上皮细胞转化序列2基因的表达及其临床意义

Expression and clinical significance of epithelial cell transforming sequence 2 gene in hepatocellular carcinoma tissues

目的探讨肝癌组织上皮细胞转化序列2(ECT2)基因的表达及其临床意义。方法从GEO数据库下载相关芯片数据,选取GPL3921平台包含的220例正常肝组织和215例肝癌组织及其附带临床信息进行分析。根据ECT2基因(219787_s_at)表达值的中位数(5. 54)将肝癌患者分为ECT2基因低表达组107例和ECT2基因高表达组108例,比较两组患者的临床特征以及预后。采用Cox模型分析肝癌患者预后的影响因素。结果肝癌组织中ECT2基因表达水平高于正常肝组织(P <0. 05);相比于ECT2基因低表达组,ECT2基因高表达组的甲胎蛋白(AFP)水平、原发肿瘤-区域淋巴结-远处转移(TNM)分期和肿瘤转移风险更高,肿瘤直径更大,预后更差(P <0. 05)。多因素Cox分析结果显示,AFP>300 ng/ml、TNM分期Ⅱ～Ⅲ期、高转移风险、ECT2基因高表达均是患者预后的独立危险因素(均P <0. 05)。结论 ECT2基因在肝癌组织中高表达,是肝癌患者预后的独立危险因素,ECT2基因高表达患者预后较差。

Objective To investigate the expression and clinical significance of epithelial cell transforming sequence 2(ECT2)gene in hepatocellular carcinoma tissues.Methods The related chip data were downloaded from the GEO database.Totally 220 cases of normal liver tissues and 215 cases of hepatocellular carcinoma tissues from GPL3921 platform as well as their clinical data were enrolled in the study.The patients with hepatocellular carcinoma were divided into low-expression ECT2 gene group(n=107)and high-expression ECT2 gene group(n=108)according to the median(5.54)expression level of ECT2 gene(219787_s_at).The clinical features and prognosis were compared between the two groups.The factors influencing the prognosis of patients with hepatocellular carcinoma were analyzed using the Cox model.Results The expression level of ECT2 gene in hepatocellular carcinoma tissues was higher than that in normal liver tissues(P<0.05);compared to the low-expression ECT2 gene group,the high-expression ECT2 gene group had higher alpha-fetoprotein(AFP)level,higher tumor-node-metastasis(TNM)stage,higher risk of tumor metastasis,greater tumor diameter and poorer prognosis(P<0.05).The results of multivariate Cox analysis showed that AFP>300 ng/ml,TNM stageⅡ-Ⅲ,high risk of metastasis,and high expression of ECT2 gene were independent risk factors for prognosis(all P<0.05).Conclusion ECT2 gene is highly expressed in hepatocellular carcinoma tissues and is an independent risk factor for prognosis of patients with hepatocellular carcinoma.Patients with high expression of ECT2 gene have a poorer prognosis.