摘要
利用双抗体夹心-酶联免疫吸附试验(double antibody sandwich enzymelinked immunosorbent assay,DAS-ELISA)和反转录-聚合酶链式反应(reverse transcription-polymerase chain reaction,RT-PCR)方法对来自土耳其的番茄种子进行种传病毒检测。结果表明,在该批番茄种子中检测到番茄斑萎病毒(Tomato spotted wilt virus,TSWV),对PCR产物进行测序,扩增片段与其他TSWV分离物的核苷酸序列相似度为99%~100%,证实该批番茄种子携带TSWV。
The tomato seeds from Turkey were detected by DAS-ELISA and RT-PCR.The results showed that Tomato spotted wilt virus(TSWV)was detected in this batch of tomato seeds.Sequencing results of PCR products showed that the nucleotide sequence similarity of the fragment to the other TSWV isolates was 99%-100%.TSWV was confirmed in this batch of tomato seeds.
作者
余辛
冯黎霞
章柱
武目涛
李新浩
丁钿
YU Xin;FENG Li-xia;ZHANG Zhu;WU Mu-tao;LI Xin-hao;DING Tian(Guangzhou Airport Entry-Exit Inspection and Quarantine Bureau,Guangzhou 510000,Guangdong,China;Inspection and Quarantine Technology Center of Guangdong Province,Guangzhou 510000,Guangdong,China)
出处
《中国蔬菜》
北大核心
2018年第11期18-21,共4页
China Vegetables
基金
科技部出入境检验检疫安全多源信息采集与融合技术项目(2018YFC0809101)