新疆阿魏乙酸乙酯部位提取物对结肠癌模型小鼠免疫功能的影响

Effects of the Extract from Ethyl Acetate Parts of Ferula sinkiangensis on the Immune Function of Colon Cancer Model Mice

目的:研究新疆阿魏乙酸乙酯部位提取物对结肠癌模型小鼠免疫功能的影响,为新疆阿魏属植物的进一步开发利用提供参考。方法:体外接种小鼠结肠癌CT-26.WT细胞复制小鼠结肠癌模型。将造模的50只小鼠随机分为模型组(生理盐水,灌胃)、顺铂组(5 mg/kg,腹腔注射)和新疆阿魏乙酸乙酯部位提取物低、中、高剂量组(按生药量计分别为1.0、2.0、3.0 g/kg,灌胃),每组10只;另选10只正常小鼠作为空白组(生理盐水,灌胃)。顺铂组小鼠隔天给药1次,其余各组小鼠均每天给药1次,连续给药10 d。给药结束后,测定各组小鼠抑瘤率和脾指数;采用流式细胞术测定各组小鼠血液中T淋巴细胞亚群CD3^+、CD4^+、CD8^+以及B淋巴细胞CD19^+的数量,并计算CD4^+/CD8^+值;酶联免疫吸附法测定小鼠血清中免疫球蛋白M(IgM)、IgG的含量。结果:与空白组比较,模型组小鼠脾指数显著升高(P<0.01),但血液中CD3^+、CD4^+、CD8^+、CD19^+细胞的数量以及血清中IgM、IgG含量差异无统计学意义(P>0.05)。与模型组比较,新疆阿魏乙酸乙酯部位提取物各剂量组小鼠的瘤质量均显著降低(P<0.01),抑瘤率均在40%以上;新疆阿魏乙酸乙酯部位提取物中、高剂量组小鼠脾指数以及血液中CD3^+细胞数量、CD4^+细胞数量、CD4^+/CD8^+值和血清中IgM、IgG含量均显著升高(P<0.05或P<0.01);新疆阿魏乙酸乙酯部位提取物各剂量组小鼠血液中CD19^+细胞数量显著减少(P<0.05或P<0.01),但血液中CD8^+细胞数量差异均无统计学意义(P>0.05)。结论:新疆阿魏乙酸乙酯部位提取物对结肠癌模型小鼠具有一定的改善作用,并可显著提高小鼠自身的细胞免疫能力和体液免疫能力。

OBJECTIVE:To study the effects of the extract from ethyl acetate part of Ferula sinkiangensis on immune function of colon cancer model mice,and to provide reference for further development and utilization of plants of the genus Ferula.METHODS:Colon cancer model mice were induced by incubated with colon cancer CT-26.WT cell.50 model mice were randomly divided into model group(normal saline,i.g.),cisplatin group(5 mg/kg,i.p.)and ethyl acetate part of F.sinkiangensis low-dose,medium-dose and high-dose groups(1.0,2.0,3.0 g/kg,calculated by crude drug,i.g.),with 10 mice in each group.Other 10 normal mice were included in blank group(normal saline,i.g.).Cisplatin group was given medicine every other day,and other groups were given medicine once a day,for consecutive 10 d.After medication,the inhibition rate of tumor and spleen index were determined.Flow cytometry was adopted to detect the number of blood T lymphocyte subsets CD3+,CD4+,CD8+and B lymphocytes CD19+;CD4+/CD8+value was calculated.ELISA method was adopted to detect the content of immune protein IgM and IgG in serum of mice.RESULTS:Compared with blank group,spleen index of mice was increased significantly in model group(P<0.01).There was no statistical significance in the number of CD3+,CD4+,CD8+and CD19+cells in the blood,as well as the contents of lgM and lgG in the serum(P>0.05).Compared with model group,the tumor mass of mice in ethyl acetate part of F.sinkiangensis groups were significantly reduced(P<0.01),inhibitory rate of tumor was more than 40%.The spleen index,the number of CD3+and CD4+,CD4+/CD8+value,the contents of IgM and IgG in serum were increased significantly in ethyl acetate part of F.sinkiangensis medium-dose and high-dose groups(P<0.05 or P<0.01).The number of CD19+cells in blood was decreased significantly in ethyl acetate part of F.sinkiangensis groups(P<0.05 or P<0.01).There was no statistical significance in the number of CD8+cells in blood(P>0.05).CONCLUSIONS:The extract from ethyl acetate part of F.sinkiangensis shows inhibitory effect on colon cancer model mice,and can significantly improve cellular immunity function and humoral immunity function.