细胞膜色谱研究进展及其在中药活性成分筛选中的应用

Progress of Cell Membrane Chromatography and Its Application in Screening Active Ingredients of Traditional Chinese Medicine

细胞膜色谱法作为近年来发展迅速的生物色谱技术,已成为筛选中药复杂体系中活性成分和研究药物与受体相互作用的重要工具。本文综述了细胞膜色谱技术的发展、分离原理、制备方法、针对其稳定性和寿命等问题进行的制备方法学优化、多种二维色谱方法学和应用策略,以及在中药等复杂体系活性成分筛选中的应用情况。在此基础上,本文展望了细胞膜色谱技术未来的发展趋势,以期为细胞膜色谱技术的进一步发展以及在药物高通量筛选领域的拓展应用提供思路。

Cell membrane chromatography,which has been used to build an in vitro screening model,has become a practical method to screen active ingredients of traditional Chinese medicine or to study the interaction between drugs and receptors.In this paper,the development of cell membrane chromatography technology and its preparation methods,the optimization of the system and methodological for its stability and other issues were reviewed,as well its unique advantages on selection of active ingredients of traditional Chinese medicine.On this basis,the future development trend of cell membrane chromatography technology was discussed in order to provide ideas for the further development of cell membrane chromatography and its further application in the field of drug high throughput screening.