初生期和快速生长期梅花鹿茸蛋白表达谱差异分析

Analysis of Protein Expression Profiles of Sika Deer Antler at Nascent and Rapid Growth Stages

采用同位素标记蛋白质相对和绝对定量技术、液相色谱-质谱联用技术和生物信息学分析方法对初生期(15 d)和快速生长期(60 d)东北梅花鹿茸进行蛋白表达谱差异分析,共鉴定出127种差异表达蛋白。其中,与快速生长期鹿茸相比,初生期鹿茸中显著性上调差异表达蛋白49种,显著性下调差异表达蛋白78种。这些差异表达蛋白主要为细胞外基质成分及参与调控神经生长和血液循环进程的功能蛋白。初生期鹿茸主要富含细胞外基质蛋白成分,且在快速生长期鹿茸中表达量显著下调。同时,与初生期鹿茸相比,参与调控神经生长和血液循环的相关蛋白表达量在快速生长期鹿茸中显著上调。这些差异表达蛋白共同参与调控梅花鹿茸的生长速度。本研究结果为进一步揭示鹿茸生长机制提供了基础数据,对研究鹿茸的临床应用具有重要的参考意义。

The protein expression profiles of the northeast Chinese Sika deer antler at nascent stage(15 d)and rapid growth stage(60 d)were investigated by using the isotope labeled protein relative and absolute quantification(iTRAQ),liquid chromatography-mass spectrometry and bioinformatics analysis.A total of 127 kinds of differentially expressed proteins were identified.Compared to rapid growth stage,there were 49 kinds of differentially expressed proteins significantly up-regulated at rapid growth stage,and there were 78 kinds of differentially expressed proteins significantly down-regulated.These differentially expressed proteins were mainly extracellular matrix components and involved in the regulation of nerve growth and blood circulation process.Among them,a variety of extracellular matrix proteins were synthesized at nascent stage,however,the expression of these proteins was significantly decreased at rapid growth stage.Meanwhile,the expression of functional proteins involved in the regulation of nerve growth and blood circulation was significantly up-regulated.These differentially expressed proteins coordinated to regulate the growth rate of Sika deer antler.The results provide the basic data for further revealing the growth mechanism,and important guiding for the clinical application.