紫杉醇联合曲安奈德局部注射治疗兔耳增生性瘢痕的疗效观察

Therapeutic Effect of Paclitaxel Combined with Triamcinolone Acetonide Local Injection in Hyperplastic Scars of Rabbit Ears

目的:研究紫杉醇联合曲安奈德局部注射对兔耳瘢痕的疗效观察。方法:选取共6只健康白色家兔,于兔耳腹侧建立动物瘢痕模型,共48个创面,共分4组依次为:空白对照组(A组)、曲安奈德治疗组(B组)、紫杉醇治疗组(C组)、紫杉醇联合曲安奈德治疗组(D组)。于术后第30天开始每组按计划局部注射药物治疗,术后第60天切取瘢痕标本,行HE染色与Caspase-3免疫组化检测,测量瘢痕指数、微血管密度及Caspase-3阳性表达以检测药物治疗兔耳瘢痕疗效及抑瘢痕机制。结果:D组明显抑制了表皮增生、真皮层成纤维细胞的增殖以及细胞外基质的积累,微血管较其他组稀疏,Caspase-3释放最多。结论:紫杉醇联合曲安奈德局部注射对兔耳瘢痕疗效更好。

Objective:The effect of paclitaxel combined with triamcinolone acetonide local injection in hyperplastic scars of rabbit ears and its related mechanism.Methods:A rabbit ear hyperplastic scar model was established.Rabbits are divided into 4 groups,included Blank control group A,triamcinolone acet-onide treatment group B,Paclitaxel treatment group C and Paclitaxel combined with triamcinolone acetonide treatment group D.Thirtieth days after the operation,each group was treated by plan.And specimens were cut on sixtieth days after operation tested hyperplastic scars,blood vessels density and caspase-3 expression of rabbit ear scars by HE staining and Immunohistochemical analysis.Results:Group D significantly inhibited the proliferation of epidermis the proliferation of dermal fibroblasts and the accumulation of extracellular matrix.Themicrovessels were more sparse than other group,and the Caspase-3 release was the most.Conclusion:The effect of paclitaxel combined with triamcinolone acetonide local injection in hyperplastic scars of rabbit ears was significant.