miR-342-3p有助于人脂肪来源间充质干细胞成脂分化过程

miR-342-3p promotes adipogenic differentiation of adipose-derived mesenchymal stem cells

目的探讨miR-342-3p对人脂肪来源的间充质干细胞(h AMSCs)向成脂分化的影响。方法利用RT-qPCR在诱导h AMSCs成脂分化过程中的不同时期检测miR-342-3p的表达。分别利用miR-342-3p基因模拟物及抑制物转染h AMSCs,诱导成脂分化后用油红O染色法观察细胞内脂滴形成情况,RT-qPCR检测PPARγ、C/EBPα和LPL mRNA的表达,Western blot检测PPARγ、C/EBPα和LPL蛋白的表达。结果 miR-342-3p在h AMSCs成脂分化的过程中表达升高,miR-342-3p模拟物转染组脂肪滴的形成数量增加,PPARγ、C/EBPα和LPL的mRNA和蛋白表达水平均显著升高(P<0. 05)。miR-342-3p抑制物转染组脂肪滴的形成数量减少,PPARγ、C/EBPα和LPL的mRNA和蛋白表达水平均显著降低(P<0. 05)。结论 miR-342-3p能够正向调节h AMSCs成脂分化。

Objective To investigate the mechanism by which miR-342-3p regulates adipogenic differentiation of human adipose-derived mesenchymal stems cells(hAMSCs).Methods hAMSCs were obtained from human adipose tissues.Adipogenic differentiation was induced by adipogenic medium,and determined by Oil Red O staining.The expression of miR-342-3p was detected by RT-qPCR.Then these cells were transfected with miR-342-3p mimics and miR-342-3p inhibitors in the test group and a negative control sequence in the control group.After adipogenic induction,RT-qPCR and Western blot were used to detect the expressions of PPARγ,C/EBPαand LPL.Results The expression of miR-342-3p was significantly elevated during adipogenic differentiation in hAMSCs.miR-342-3p overexpression remarkably promoted hAMSCs differentiate into adipogenic lineage,as indicated by significant increase in the amount of lipid droplets compared with negative control transfected cells.Moreover,the mRNA and protein levels of PPARγ,C/EBPαand LPL were all upregulated significantly(P<0.05).Adipogenesis was significantly blocked by miR-342-3p downregulation.Conclusions miR-342-3p is a differentiation enhancer of the adipogenesis of hAMSCs.