长链非编码RNA GASL1在乳腺癌组织及细胞系中的表达及作用

Expression and effect of lncRNA GASL1 in breast cancer tissues and cell line

目的探讨长链非编码RNA(lncRNA) GASL1在乳腺癌中的表达及其对乳腺癌癌细胞增殖和凋亡的影响。方法RT-qPCR检测癌组织、癌旁组织、多个乳腺癌细胞系(MDA-MB-231、MDA-MB-468和SK-BR-3)及正常乳腺细胞HBL-100中lncRNA GASL1的表达水平。MCF-7细胞经pc DNA3. 1质粒过表达lncRNA GASL1,或siRNA干扰lncRNA GASL1表达后,运用MTT法检测细胞增殖; caspase-3活力检测试剂盒检测caspase-3活力; Western blot检测Bax和Bcl-2蛋白表达。结果 LncRNA GASL1在乳腺癌组织和乳腺癌细胞中表达水平降低(P <0. 05);过表达lncRNA GASL1可抑制MCF-7的增殖,促进其凋亡(P<0. 05);干扰lncRNA GASL1表达可促进MCF-7的增殖,抑制其凋亡(P<0. 05)。结论 lncRNA GASL1在乳腺癌中表达水平降低,通过调节乳腺癌细胞增殖和凋亡参与乳腺癌的发生发展。

Objective To investigate the expression of lncRNA GASL1 in breast cancer and the effect of lncRNA GASL1 on the proliferation and apoptosis of breast cancer.Methods RT-qPCR was used to measure the expression of lncRNA GASL1 in breast cancer tissue and cells.pcDNA3.1 plasmid and siRNA were used to over-express and knockdown the expression of lncRNA GASL1 respectively in MCF-7 cells,and then measured the proliferation through MTT method,caspase 3 Activity Assay Kit was used to measure the caspase 3 activity.Moreover,the protein levels of Bax and Bcl-2 were tested by Western blot.Results Lower lncRNA GASL1 level in breast cancer tissue and cells as compared with paracancerous normal tissues and normal breast cell HBL-100,respectively(P<0.05).Overexpression of lncRNA GASL1 inhibited MCF-7 proliferation and promoted apoptosis(P<0.05),while knockdown of lncRNA GASL1 increased proliferation and suppressed apoptosis(P<0.05).Conclusions The expression of lncRNA GASL1 is lower in breast cancer,which is involved in development of breast cancer through the regulation on breast cancer cell proliferation and apoptosis.