摘要
目的探讨长链非编码RNA(lncRNA) GASL1在乳腺癌中的表达及其对乳腺癌癌细胞增殖和凋亡的影响。方法RT-qPCR检测癌组织、癌旁组织、多个乳腺癌细胞系(MDA-MB-231、MDA-MB-468和SK-BR-3)及正常乳腺细胞HBL-100中lncRNA GASL1的表达水平。MCF-7细胞经pc DNA3. 1质粒过表达lncRNA GASL1,或siRNA干扰lncRNA GASL1表达后,运用MTT法检测细胞增殖; caspase-3活力检测试剂盒检测caspase-3活力; Western blot检测Bax和Bcl-2蛋白表达。结果 LncRNA GASL1在乳腺癌组织和乳腺癌细胞中表达水平降低(P <0. 05);过表达lncRNA GASL1可抑制MCF-7的增殖,促进其凋亡(P<0. 05);干扰lncRNA GASL1表达可促进MCF-7的增殖,抑制其凋亡(P<0. 05)。结论 lncRNA GASL1在乳腺癌中表达水平降低,通过调节乳腺癌细胞增殖和凋亡参与乳腺癌的发生发展。
Objective To investigate the expression of lncRNA GASL1 in breast cancer and the effect of lncRNA GASL1 on the proliferation and apoptosis of breast cancer.Methods RT-qPCR was used to measure the expression of lncRNA GASL1 in breast cancer tissue and cells.pcDNA3.1 plasmid and siRNA were used to over-express and knockdown the expression of lncRNA GASL1 respectively in MCF-7 cells,and then measured the proliferation through MTT method,caspase 3 Activity Assay Kit was used to measure the caspase 3 activity.Moreover,the protein levels of Bax and Bcl-2 were tested by Western blot.Results Lower lncRNA GASL1 level in breast cancer tissue and cells as compared with paracancerous normal tissues and normal breast cell HBL-100,respectively(P<0.05).Overexpression of lncRNA GASL1 inhibited MCF-7 proliferation and promoted apoptosis(P<0.05),while knockdown of lncRNA GASL1 increased proliferation and suppressed apoptosis(P<0.05).Conclusions The expression of lncRNA GASL1 is lower in breast cancer,which is involved in development of breast cancer through the regulation on breast cancer cell proliferation and apoptosis.
作者
吴多明
武力
张晓斌
马大昌
WU Duo-ming;WU Li;ZHANG Xiao-bin;MA Da-chang(Dept.of Breast Diseases,the First Hospital of Lanzhou University,Lanzhou 730000,China)
出处
《基础医学与临床》
CSCD
2018年第11期1543-1547,共5页
Basic and Clinical Medicine