屈头鸡果预灌胃对哮喘小鼠气道Th1/Th2型细胞因子水平的调节作用

Regulatory effect of Capparis versicolor Griff on airway type Th1/Th2 cytokines in asthmatic mice

目的观察屈头鸡果预灌胃对哮喘小鼠气道Th1/Th2型细胞因子水平的调节作用。方法将50只BALB/c小鼠随机分为正常对照组(A组)、哮喘模型组(B组)、屈头鸡果治疗剂量组(C组)、屈头鸡果中毒剂量组(D组)、地塞米松治疗组(E组) 5组,每组10只。B组第0、14天腹腔注射0. 2 m L致敏液,第24天以1%卵蛋白溶液10 m L雾化激发哮喘,每天30 min,连续28天; A组代之等量生理盐水; C、D组以上述同样方法用卵蛋白进行致敏和激发小鼠,在每次雾化激发哮喘前30 min分别灌胃屈头鸡果果浆2. 16、10. 8 g/kg,E组腹腔注射地塞米松2mg/kg。在末次激发结束后采集各组小鼠肺泡灌洗液,以ELISA法测定各组小鼠肺泡灌洗液Th1型细胞因子IL-2、IL-10、IFN-γ以及Th2型细胞因子IL-4、IL-5的水平。结果末次激发结束时A组、B组、C组及E组小鼠均存活,D组小鼠在给药的过程中先后死亡4只,存活的6只也存在不同程度的呕吐、腹泻、心律加快和抽搐等症状。与A组比较,B、C、D、E组小鼠肺泡灌洗液IL-2、IL-10、IFN-γ水平低,IL-4、IL-5水平高(P均<0. 01)。与B组比较,C、D、E组小鼠肺泡灌洗液IL-2、IL-10、IFN-γ水平高,IL-4、IL-5水平低(P均<0. 05)。结论屈头鸡果预灌胃对哮喘小鼠气道IL-4、IL-5等Th2型细胞因子水平具有抑制作用,并可激活IL-2、IL-10、IFN-γ等Th1型细胞因子,具有抑制哮喘气道炎症的作用;但屈头鸡果具有一定毒性。

Objective To observe the regulatory effect of Capparis versicolor Griff on airway type Th1/Th2 cytokines in asthmatic mice.Methods Fifty BALB/c mice were randomly divided into five groups:normal control group(group A),asthma model group(group B),Capparis versicolor Griff therapeutic dose group(group C),Capparis versicolor Griff toxic dose group(group D),and dexamethasone treatment group(group E).Group B was administered 0.2 mL sensitizer intraperitoneally on the 0 and 14th day,and the asthma was stimulated by aerosol inhalation of 1%ovalbumin solution(10 mL)on the 24th day for 30 minutes every day,for 28 days;group A was replaced by the same amount of normal saline;groups C and D were sensitized and stimulated by ovalbumin in the same way,and the mice were given Capparis versicolor Griff 2.16 g/kg(group C)or 10.8 g/kg(group D)30 minutes before every atomization;group E was intraperitoneally injected with dexamethasone 2 mg/kg.After the last stimulation,alveolar lavage fluid was collected and the levels of Th1 cytokines interleukin-2(IL-2),IL-10,tumor necrosis factor-γ(IFN-γ),Th2 cytokines IL-4 and IL-5 in the alveolar lavage fluid were determined by enzyme linked immunosorbent assay(ELISA).Results At the end of the last stimulation,mice in the group A,group B,group C and group E all survived.Four mice in the group D died successively in the course of administration.Six survived mice also suffered from vomiting,diarrhea,tachycardia and convulsions.Compared with the group A,the levels of IL-2,IL-10 and IFN-γin the alveolar lavage fluid were lower,and the levels of IL-4 and IL-5 were higher in the groups B,C,D and E(all P<0.01).Compared with the group B,the levels of IL-2,IL-10,and IFN-γwere higher,and the levels of IL-4 and IL-5 were lower in the groups C,D and E(all P<0.05).Conclusion The pretreatment of Capparis versicolor Griff can inhibit the levels of Th 2 cytokines such as IL-4 and IL-5 in the airway of asthmatic mice,and activate Th1 cytokines such as IL-2,IL-10 and IFN-gamma,which can inhibit the airway inflammation of asthma,but Capparis versicolor Griff has certain toxicity.