春榨菜细胞质雄性不育系09-05A的形态学和细胞学特征

Morphological and cytological characteristics of cytoplasmic male sterility system 09-05A in spring stem mustard(Brassica juncea var.tumida Tsen et Lee)

榨菜不育系09-05A是由欧新A雄性不育源转育而成的新型春榨菜雄性不育系。本研究通过扫描电镜和透射电镜等对春榨菜雄性不育系09-05A在不同发育时期的花药和花粉进行形态学和细胞学观察发现,小孢子从单核时期开始发生异常,并随着花粉发育的进行,细胞内含物不断降解,最终形成空瘪、畸形的花粉粒。本研究阐明了不育系09-05A花粉败育的发生时期和细胞学特征,为深入进行榨菜雄性不育的发育机制研究提供了理论依据。

Cytoplasmic male sterility(CMS)09-05A is a new CMS line in spring stem mustard that is transferred from oxa A CMS.In this study,morphological and cytological observation of anther and pollen grain of 09-05A CMS was carried out by scanning electron microscopy and transmission electron microscope.It was revealed that the microspore abortion were initiated at the uninucleate stage.The cell nucleus and other cellular components of microspores were degraded to form collapsed and abortive pollen grains eventually during the pollen development.This study clarified the abortive stage and cytological characteristics,which may help to uncover the developmental mechanism of CMS 09-05A and provide a theoretical basis for the heterosis utilization of CMS in stem mustard(Brassica juncea var.tumida Tsen et Lee).