转PeTLP基因‘南林895’杨对土壤微生物的影响及外源基因分子检测

Effect of Transgenic Populus deltoides ×P. euramericanna cv. 'Nanlin 895' with PeTLP on Soil Microbes and Molecular Analysis on Exogenous Genes

转基因技术是当今林木分子育种的手段之一,但其生物安全性问题广受关注。本研究选择进入田间试验的转PeTLP(类甜蛋白)基因‘南林895’杨Populus deltoides×P. euramericana cv.‘Nanlin 895’为材料,开展转基因杨树对土壤微生物影响及外源基因分子检测等分析。结果显示,转基因杨树在进入田间试验一年后,外源基因仍稳定存在于转基因植株基因组中;转基因与非转基因植株根系周围可培养土壤微生物菌落数量无显著差异,表明外源基因对土壤微生物无显著影响;对土壤微生物总DNA进行分子检测也显示外源基因未有向周围土壤微生物扩散现象;叶片化感作用试验显示转基因植株叶片未对生菜Lactuca sativa L. var. ramose Hort.种子的生长造成显著影响。初步分析结果表明,转PeTLP基因‘南林895’杨在进入田间试验后未出现外源基因水平转移,也未对周围生态环境造成显著影响。

Transgenic Populus deltoides×P.euramericana cv.‘Nanlin 895’with PeTLP gene were selected for molecular analysis and biosafety analysis.The results showed that the exogenous gene could be detected after one year plantation of transgenic poplars in the open field.There was no significant difference in the quantity of soil microbes around root system between transgenic and non-transgenic plants,indicating that the transgenic plants with PeTLP had no significant effects on soil microbes.Molecular analysis on total DNA of soil microbes showed that the exogenous gene did not transfer to the soil microbes.Allelopathy effect of leaf of transgenic‘Nanlin 895’showed that it had no significant effect on the seed growth of Lactuca sativa L.var.ramose Hort.