达氟沙星对猪胸膜肺炎放线杆菌的体外药效研究

Study on the Effect of Danofloxacin on Actinobacillus pleuropneumonia in vitro

为了解猪胸膜肺炎放线杆菌对达氟沙星的敏感性和达氟沙星的杀菌效果,收集和分离48株猪胸膜肺炎放线杆菌,采用微量肉汤稀释法和菌落计数法对体外药物敏感性试验和生长曲线、杀菌曲线进行了研究。猪胸膜肺炎放线杆菌对氨苄西林、磺胺异恶唑、头孢噻呋、大观霉素、黏菌素耐药率高,对阿莫西林/克拉维酸、复方新诺明、氟苯尼考、多西环素、庆大霉素、四环素、恩诺沙星、氧氟沙星、达氟沙星敏感。达氟沙星最小抑菌浓度(MIC)集中在0.0078μg/m L。达氟沙星浓度在MIC及以上时,随浓度升高,杀菌时间缩短,为典型的浓度依赖型抗生素。该试验结果可用于指导猪传染性胸膜肺炎的临床预防和治疗。

To understand the sensitivity of Actinobacillus pleuropneumonia to danofloxacin and the effect of danofloxacin,48 strains are selected and isolated to investigate the drug sensitivity test,growth curve and killing curve in vitro by micro broth dilution method and colony counting method.The results showed that the pathogen are resistant to ampicillin,sulfamethoxazole,ceftiofur,spectinomycin and colistin and sensitive to amoxicillin/clavulanic acid,compound sulfamethoxazole/TMP,florfenicol,doxycycline,gentamicin,tetracycline,enrofloxacin,ofloxacin and danofloxacin.The minimum inhibitory concentration of danofloxacin is 0.0078μg/mL.When the concentration of danofloxacin is at MIC and above,the sterilization time is shortened with the increase of concentration.It is a typical concentration dependent antibiotic.These findings can assist in the clinical prevention and the treatment of porcine contagious pleuropneumonia.