酵母静息细胞耦合原位分离技术连续转化2-苯乙醇

Continuous bioconversion of 2-phenylethanol by yeast resting cells coupled with in situ product removal technique

以酵母静息细胞作为酶载体,大孔吸附树脂为分离介质,微滤膜分离菌体,采用原位产物分离技术进行连续转化合成2-苯乙醇(2-phenylethanol,简称2-PE)。在5 L发酵罐中优化了转化条件,通过补料和控制产物浓度实现连续转化合成2-苯乙醇。单罐运行144 h,2-苯乙醇产量达24. 06 g/L,摩尔转化率和平均转化速率分别为79. 34%和0. 166 g/(L·h)。双罐运行144 h,2-苯乙醇产量达29. 86 g/L,比单罐产量提高24. 1%;摩尔转化率和平均转化速率分别达81. 83%和0. 204 6 g/(L·h),静息细胞重复使用10批次,2-PE产量和平均转化速率没有明显下降。

Using the yeast resting cells and macroporous adsorptive resins respectively as enzyme carriers and separation medium,continuous transformation of 2-phenylethanol was carried out with the aid of in situ product removal techniques.Meanwhile,the strains were separated through microfiltration membrane.The transformation conditions were optimized in 5 L fermentor,and continuous bioconversion of 2-phenylethanol was fulfilled by feeding and controlling product concentration.The concentration of 2-phenylethanol was 24.06 g/L after running 144 h in single tank.The molar conversion rate was 79.34%,and the average space-time yield was 0.166 g/(L·h).After running 144 h in double tanks,the concentration of 2-phenylethanol reached 29.86 g/L,which was higher than single tank production by 24.1%.The molar conversion rate and the average space-time yield were up to 81.83%and 0.204 6 g/(L·h),respectively.After repeated use of resting cells for 10 times,the production and average conversion rate of 2-PE did not decrease obviously.