长链非编码RNA肺腺癌转移相关转录本1在肝细胞癌患者血浆中的表达及其临床意义研究

Plasma Expression and Clinical Significance of the lncRNA MALAT1 in Patients with Hepatocellular Carcinoma

目的探讨长链非编码RNA肺腺癌转移相关转录本1(MALAT1)在肝细胞癌(HCC)患者血浆中的表达及临床意义。方法收集2015年4月—2017年4月就诊于新疆医科大学第一附属医院,经病理诊断为HCC的120例患者的血浆及血清样本。采用实时荧光定量聚核酶链式反应(qRT-PCR)检测HCC患者血浆MALAT1表达水平,应用酶联免疫吸附试验(ELISA)检测血清基质金属蛋白酶(MMP)9、血管内皮生长因子(VEGF)水平。以血浆MALAT1表达水平的中位数将患者分为MALAT1高、低表达组,分析血浆MALAT1表达水平与HCC患者临床特征及血清VEGF、MMP9表达水平的关系。结果 MALAT1高、低表达组肿瘤分期、转移情况比较,差异有统计学意义(P<0.05);MALAT1高、低表达组性别、年龄、Child-Pugh分级、血清甲胎蛋白(AFP)表达水平、病理分级、HBV感染情况、肝硬化情况比较,差异无统计学意义(P>0.05)。COX多因素回归分析结果显示,年龄、肿瘤分期、Child-Pugh分级、有无转移、血浆MALAT1表达水平是HCC患者预后的独立影响因素(P<0.05)。MALAT1高、低表达组生存率比较,差异有统计学意义(P<0.010)。血浆MALAT1表达水平与血清MMP9、VEGF表达水平呈正相关(P<0.010)。结论血浆MALAT1高表达的HCC患者生存期短,容易出现转移。

Objective To investigate the expression and clinical significance of metastasis associated lung adenocarcinoma transcript 1(MALAT1),a long non-coding RNA,in the plasma of patients with hepatocellular carcinoma(HCC).Methods We collected 120 plasma and 120 serum samples from pathologically diagnosed HCC patients admitted to the First Affiliated Hospital of Xinjiang Medical University between April 2015 and April 2017.The expression of MALAT1 in the peripheral blood plasma of the HCC patients was detected by real-time fluorescence quantitative polymerase chain reaction(RTqPCR).Serum MMP9 and VEGF levels were detected by enzyme-linked immunosorbent assay.The patients were divided into high and low expression groups with the median value of MALAT1 as the cut point,and the correlations between MALAT1 expression and clinical characteristics as well as the serum MMP9 and VEGF level were analyzed.Results There were significant differences in tumor stage and metastasis between the MALAT1 high and low expression groups(P<0.05).Other clinical characteristics including sex,age,Child-Pugh classification,serum AFP level,pathological grade,HBV infection and cirrhosis conditions were not significantly different between the two groups(P>0.05).Cox multivariate regression analysis showed that age,tumor stage,Child-Pugh classification,metastasis,plasma MALAT1 expression levelwere independent risk factors affecting the prognosis of HCC patients(P<0.05).Survival analysis showed that the survival rates in the MALAT1 high and low expression groups were significantly different(P<0.010).Spearman correlation analysis showed that the expression level of MALAT1 was positively correlated with the levels of MMP9 and VEGF(P<0.010).Conclusion Patients with high expression of plasma MALAT1 have a short survival time and are prone to metastasis.