木霉菌协助丛枝菌根真菌对煤矸石分解和绿化的促进效果

Promotion Effect of Arbuscular Mycorrhizal Fungi Assisted by Trichodermaon Coal Gangue Decomposition and Revegetation

为了研究木霉菌协助丛枝菌根真菌对煤矸石的分解与绿化效果的作用,将煤矸石中单施1.5%的丛枝菌根真菌处理,低、中、高三种剂量(0.03%,0.15%,0.30%)的木霉菌分别与1.5%的丛枝菌根真菌混施处理,通过培养皿降解实验和盆栽紫花苜蓿实验进行对比研究,测定了煤矸石的分解率、绿化效果指标以及煤矸石基质的养分、微生物指标.结果表明:单施丛枝菌根真菌能够增加煤矸石的速效磷钾、微生物量碳氮磷、根外菌丝长度,提高过氧化氢酶和磷酸酶活性,最终促进煤矸石的分解和绿化.不同剂量的木霉菌与丛枝菌根真菌混施后,与单施丛枝菌根真菌相比,煤矸石基质的上述指标均有不同程度的提高,最终进一步促进了煤矸石的分解和绿化.其中中剂量的木霉菌协助促进效果最好,使煤矸石分解率、紫花苜蓿的菌根侵染率和紫花苜蓿鲜重分别显著提高了37.98%,39.70%和39.77%,而低剂量和高剂量木霉菌协助促进效果不显著.因此,中剂量木霉菌可协助丛枝菌根真菌促进煤矸石的分解和绿化,具有良好的效果.

In order to study the composition and revegetation effect of Arbuscular mycorrhizal fungi(AMF)assisted by Trichoderma on coal gangue,treatment of applying 1.5%Arbuscular mycorrhizal fungi alone in coal gangue,treatment of applying mixture of low,medium and high dose(0.03%,0.15%,0.30%)of Trichoderma spp.respectively and 1.5%AMF in coal gangue were comparatively studied through the Petri dish degradation experiment and the pot alfalfa experiment.The rate of decomposition of coal gangue,indicators of revegetation effectiveness,and nutrient and microbial indicators of coal gangue matrix were determined.The results show that application of AMF alone can increase available P and K content,microbial biomass C,N,P,extraradical hyphae length,enhance activities of catalase and phosphatase,and enventually promote decomposition and revegetation of coal gangue.Compared with application of AMF alone,AMF mixed with different dose of Trichoderma can enhance the above indicators to different degree,and enventually further promote decomposition and revegetation of coal gangue.Medium dose of Trichoderma has the best assistance effect,which significantly improves coal gangue decomposition,mycorrhizal infection rate of alfalfa and alfalfa fresh weight by 37.98%,39.70%and 39.77%,respectively.Low and high dose of Trichoderma has not significant assistance effect.Therefore,medium dose of Trichoderma can assist AMF to promote decomposition and revegetation of coal gangue with good effects.