ADAM15通过激活EGFR促进结直肠癌细胞的转移侵袭

ADAM15 promotes metastasis and invasion of colorectal cancer cells by activating EGFR

目的探讨结直肠癌细胞中ADAM15的表达及其对结直肠癌细胞迁移侵袭能力的影响。方法 Western blotting检测人正常结直肠黏膜细胞系FHC和人结直肠癌细胞系Caco-2、SW-480、DKO-1中ADAM15蛋白的表达。向SW-480细胞分别转染si NC(si NC组)和si ADAM15(si ADAM15组),细胞划痕实验和Transwell实验检测两组SW-480细胞迁移、侵袭能力; Western blotting检测si NC组、si ADAM15组和FHC细胞系中p-EGFR、EGFR、ADAM15蛋白的表达。结果 Caco-2、SW-480及DKO-1细胞系中ADAM15表达量分别为2. 01±0. 149、2. 74±0. 053、2. 27±0. 185,均高于FHC细胞系的0. 98±0. 043,差异有统计学意义(P<0. 05)。划痕实验显示,si ADAM15组24 h划痕宽度与0 h划痕宽度比值为0. 925±0. 087,明显高于si NC组的0. 326±0. 031,差异有统计学意义(P<0. 05)。Transwell实验显示,si ADAM15组SW-480细胞侵袭数目为327. 4±42. 13,显著低于si NC组的669. 7±35. 21,差异有统计学意义(P<0. 05)。si NC组SW-480细胞中p-EGFR表达量为0. 89±0. 097,显著高于FHC细胞系的0. 42±0. 035,差异有统计学意义(P<0. 05); si ADAM15组SW-480细胞中p-EGFR表达量为0. 27±0. 067,显著低于si NC组的0. 89±0. 097,差异有统计学意义(P<0. 05)。结论 ADAM15可能通过激活EGFR促进结直肠癌细胞的迁移侵袭过程。

Objective To investigate the expression of ADAM15 in colorectal cancer cells and its effect on migration and invasion of colorectal cancer cells.Methods The expression of ADAM15 protein in human normal colorectal mucosal cell line FHC and human colorectal cancer cell line Caco-2,SW-480 and DKO-1 was detected by Western blotting.SW-480 cells were transfected into siNC(siNC group)and siADAM15(siADAM15 group)respectively.Cell scratch test and Transwell test were used to detect the migration and invasion ability of the two groups.The expressions of p-EGFR,EGFR and ADAM15 proteins in siNC,siADAM15 and FHC were detected Western blotting.Results The expression levels of ADAM15 in Caco-2,SW-480 and DKO-1 cell lines were 2.01±0.149,2.74±0.053 and 2.27±0.185,respectively,which were higher than 0.98±0.043 in FHC cell line,and the difference was statistically significant(P<0.05).Scratch test showed that the ratio of 24 h scratch width to 0 h scratch width in siADAM15 group was 0.925±0.087,which was significantly higher than 0.326±0.031 in siNC group,and the difference was statistically significant(P<0.05).Transwell test showed that the invasive number of SW-480 cells in siADAM15 group was 327.4±2.13,which was significantly lower than 669.7±35.21 in siNC group(P<0.05).The expression of p-EGFR in SW-480 cells of siNC group was 0.89±0.097,which was significantly higher than 0.42±0.035 in FHC cell line,and the difference was statistically significant(P<0.05).The expression of p-EGFR in SW-480 cells of siADAM15 group was 0.27±0.067,which was significantly lower than 0.89±0.097 of siNC group,and the difference was statistically significant(P<0.05).Conclusion ADAM15 may promote the migration and invasion of colorectal cancer cells by activating EGFR.