力量训练对绝经女性骨骼肌mRNA表达谱的影响

Differential Expression and Functional Analysis of mRNAs in Skeletal Muscles Induced by Power Training in Postmenopausal Women

目的研究女性绝经1年后接受力量训练1年骨骼肌mRNA表达谱的改变情况,并探讨其潜在的功能。方法从人类基因表达汇编(GEO)数据库下载GSE16907。在对照观察组和1年期锻炼组分别进行差异表达基因分析,找出入组时和1年后骨骼肌的差别表达基因,并进行基因本体分析,探讨其所涉及的生物过程。另外,采用基因集富集分析(GSEA)方法分析与入组时相比,1年后对照组标本富集的基因通路。结果 1年后对照组绝经女性骨骼肌细胞富集了"调节端粒帽"相关通路。ATM、TERF2等基因表达升高。与1年前相比,表达上调的DEGs参与了促进脂肪细胞分化、调节成骨细胞分化、负向调节ATP代谢和负向调节辅因子代谢等生物过程。而在锻炼组中,未发现上述改变。而且,其表达上调的DEGs参与了骨骼肌收缩和糖酵解等生物过程。结论间隔1年后,绝经女性骨骼肌出现衰老或退行性的基因组学改变,力量训练可以逆转这一过程。

Objective To investigate the differences in the mRNA expression profile of skeletal muscles of postmenopausal women undergoing 1 year of power training and to determine the functions of the identified differentially expressed mRNAs.Methods GSE16907 was downloaded from the Gene Expression Omnibus(GEO)database.Differentially expressed genes between baseline and 1-year follow-up samples,in the control and the power-training group,respectively,were screened out.The resulting data were subsequently subjected to gene ontology analysis to reveal the biological processes involved.In addition,gene set enrichment analysis(GSEA)was performed in the control group to compare the enriched functional gene sets between the 1-year follow-up and baseline samples.Results In the control group,“regulating telomere capping”-related genes were enriched;the expressions of genes including ATM and TERF2 were upregulated in the skeletal muscle cells of the 1-year follow-up samples from postmenopausal women.Moreover,the DEGs were found to be involved in multiple biological processes,such as promotion of adipocyte differentiation,modification of osteoblast differentiation,and inhibition of ATP and cofactor metabolism.In the 1-year power-training group,multiple biological processes,including the skeletal muscle contraction and glycolysis,were identified by the GO-BP analysis of DEGs in the postmenopausal women.Conclusion Senile/degenerative genomic alterations occurred in the skeletal muscles of females after 1 year of menopause.However,1-year power training of the skeletal muscles could reverse this aging process in the postmenopausal women.