果糖饮食致大鼠肠内菌群的变迁及双歧杆菌干预作用的研究

Changes of intestinal flora in rats with metabolic disorders caused by fructose diet and intervention effect of Bifidobacterium

目的探究果糖饮食致大鼠代谢异常中拟杆菌属、乳杆菌属和梭杆菌属丰度变化及双歧杆菌干预对3种菌属和代谢的影响。方法将SD雄性大鼠随机分为对照组(NC组)、果糖组(HFD组)和双歧杆菌组(B组),每组各10只。NC组,普通饲料+自来水喂养;HFD组,普通饲料+10%果糖水喂养;B组,普通饲料+10%果糖水+双歧杆菌水(1 ml/d,1×109 cfu/ml)灌胃。其他饲养条件相同,每周检测大鼠体重。于16周末空腹抽血检测谷丙转氨酶(ALT)、谷草转氨酶(AST)、三酰甘油(TG)、脂多糖(LPS)、肿瘤坏死因子α(TNF-α)及空腹胰岛素(FINS)水平,计算胰岛素抵抗指数(HOMA-IR);实时聚合酶链反应(real-time PCR)检测大鼠粪便中3种菌属丰度变化;镜下观察肝组织病理学变化。结果与NC组比较,HFD组血浆LPS、ALT、AST、TG、TNF-α、空腹血糖及FINS差异有统计学意义(P <0.05),HFD组升高;粪便中拟杆菌属、乳杆菌属丰度下降,梭杆菌属丰度升高,差异有统计学意义(P <0.05);双歧杆菌干预对HFD组上述指标有一定程度的改善。结论果糖饮食引起3种菌属失调参与机体代谢异常的发生,双歧杆菌通过调节其失调对机体代谢紊乱发挥改善作用。

Objective To explore the changes of three genera in rats with metabolic disorders caused by fructose diet and the effect of Bifidobacterium on these genera and metabolism of rats.Methods Male SD rats were randomly divided into a normal control group(NC group,fed with normal diet),a high fructose diet group(HFD group,fed with normal diet and 10%fructose water)and a Bifidobacterium group(B group,fed the same as HFD group and with 1×109 cfu/ml Bifidobacterium water through intragastric administration);each group had 10 rats.Weight of the rats was monitored weekly.The levels of liver enzymes(ALT and AST)and the levels of TG,LPS,TNF-α,FBG and FINS in the plasma of the rats were measured,and HOMA-IR was calculated at the end of the 16th week.The content of Bacteroides,Lactobacillus and Fusobacterium Spp.in feces was detected via real-time PCR.The pathological changes of liver tissues in each group were observed.Results Compared with the NC group,the plasma levels of liver enzymes(ALT,AST),TG,LPS and TNF-α,FBG and FINS were increased in the HFD group(P<0.05),the relative content of Bacteroides and Lactobacillus in feces decreased(P<0.05),while the relative abundance of Fusobacterium Spp.increased(P<0.05).Compared with the HFD group,the levels of the above plasma biochemical indexes were lower in the B group(P<0.05),while the relative content of Bacteroides and Lactobacillus in feces increased(P<0.05),and the relative abundance of Fusobacterium Spp.decreased(P<0.05)in the B group.Conclusions The changes of three genera caused by fructose diet are involved in metabolic disorders,and Bifidobacterium can improve the metabolic disorders in rats by regulating them.