阴道分泌物五联检试验项目诊断效能研究

Study on diagnostic efficiency of quintuple test for vaginal secretions

目的探讨阴道分泌物爱必维(AV/BV五联检)酶化学反应法试验项目的诊断效能,为检验科质量控制和临床诊断提供一定的科学依据。方法选择东莞常安医院2017年4月1日至2017年12月31日患者1 804例,所有标本均进行AV/BV五联检酶化学反应法、细菌培养法和显微镜镜检法等检测方法,同时将患者信息和检验结果录入系统,统计5项指标灵敏度、特异度、诊断符合率、漏诊率、误诊率等诊断效能。结果1 804例患者AV/BV五联检酶化学法阳性率为26.98%,阴性率为73.02%;其中过氧化氢试验灵敏度为93.55%,特异度为95.66%,诊断符合率为94.73%,试验漏诊率为6.45%,误诊率为4.34%;唾液酸苷酶试验灵敏度为87.50%,特异度为99.03%,诊断符合率为97.34%,试验漏诊率为12.50%,误诊率为0.97%;白细胞酯酶试验灵敏度为93.22%,特异度为95.36%,诊断符合率为94.35%,试验漏诊率6.78%,误诊率4.64%;β-葡萄糖醛酸苷酶和凝固酶联合试验灵敏度为92.65%,特异度为98.11%,诊断符合率为97.28%,试验漏诊率为7.35%,误诊率为1.89%。结论阴道分泌物AV/BV五联检项目诊断效能强,可以作为细菌培养和镜检方法学的补充。

Objective To explore the diagnostic efficiency of AV/BV quintuple enzyme chemical reaction test for vaginal secretions,and to provide scientific basis for quality control and clinical diagnosis in laboratory.Methods All specimens of 1 804 patients in Dongguan Chang′an Hospital from April 1,2017 to December 31,2017 were examined by AV/BV quintuple enzyme chemical reaction method,bacterial culture method and microscopic examination.At the same time,the patient information and test results were input into the system,and the sensitivity,specificity,diagnostic coincidence rate,missed diagnosis rate,misdiagnosis rate and other diagnostic efficacy of the five indicators were statistically analyzed.Results The positive rate and negative rate of AV/BV quintuple enzyme chemical method were 26.98%and 73.02%in 1 804 patients,among which the sensitivity,specificity,diagnostic accuracy,missed diagnosis and misdiagnosis rate of H 2O 2 test were 93.55%,95.66%,94.73%,6.45%and 4.34%,respectively.The sensitivity and specificity of SNA test were 87.50%,99.03%,97.34%,12.50%and 0.97%respectively.The sensitivity and specificity of LE test were 93.22%,95.36%,94.35%,6.78%and 4.64%respectively.The sensitivity,specificity,diagnostic accuracy,missed diagnosis rate and misdiagnosis rate of the combined test of GUS and GADP were 92.65%,98.11%,97.28%,7.35%and 1.89%,respectively.Conclusion The diagnostic efficiency of vaginal secretion AV/BV quintuple test project is strong,can be used as a supplement to bacterial culture and microscopic methods.