胰岛素样生长因子1基因多态性与多囊卵巢综合征相关性研究

Association between insulin-like growth factor 1 gene polymorphism and polycystic ovary syndrome

目的分析胰岛素样生长因子1(IGF-1)基因多态性与多囊卵巢综合征(PCOS)的相关性。方法选择自2016年2月至2017年3月在我院接受治疗的274例PCOS患者作为病例组,同时选择同期250名体检健康者作为对照组,经静脉取血后收集血清后,采用自动生化分析仪分析测定相关糖脂代谢指标,包括空腹胰岛素(FINS)、低密度脂蛋白胆固醇(LDL-C)、空腹血糖(FPG)、高密度脂蛋白胆固醇(HDL-C),同时采用ELISA实验检测黄体生成素(LH)、卵泡刺激素(FSH),并计算体重指数(BMI);采用PCR-RFLP法分析IGF-1基因位点G201A、T455C的多态性;同时经Logistic回归分析讨论PCOS的危险因素。结果 PCOS组患者的BMI为(24.63±3.71)kg/m2,LDL-C为(2.23±0.38)mmol/L,FPG为(5.43±0.64)mmol/L,FINS为(9.35±1.96)m IU/L,LH为(6.49±1.23)IU/L,显著高于对照组(P<0.05);PCOS组患者的HDL-C为(1.39±0.21)mmol/L,FSH为(5.68±1.14)IU/L,显著低于对照组(P<0.05)。病例组的T455C位点TT基因型、CT基因型、CC基因型与对照组分布差异有统计学意义(P<0.05)。病例组G201A位点AA基因型、AC基因型、CC基因型与对照组差异有统计学意义(P<0.05)。经多因素Logistic回归分析,发现IGF-1基因T455C、G201A位点多态性和血清中高水平FINS是PCOS的危险因素(P<0.05)。结论 IGF-1基因多态性与PCOS发生密切相关,为患者临床治疗提供实验依据。

Objective To analyze the gene polymorphism of insulin-like growth factor-1(IGF-1)and the association with polycystic ovary syndrome(PCOS)in women.Methods 274 patients in our hospital for treatment of patients with PCOS from February 2016 to March 2017 were selected as pcos group.will 250 healthy persons were enrolled as control group.And intravenous blood serum was collected and analyzed by automatic biochemical analyzed for the determination of fasting insulin(FINS),low density lipoprotein protein cholesterol(LDL-C),fasting blood glucose(FPG)and high density lipoprotein cholesterol(HDL-C)and luteinizing hormone(LH),follicle stimulating hormone(FSH)were analyzed ELISA,and body mass index(BMI)was determined;PCR-RFLP was used to analyze IGF-1 gene polymorphism sites of G201A and T455C.And risk factors of PCOS was evaluated by using Logistic regression analysis.Results The BMI of patients in the PCOS group was(24.63±3.71)kg/m2,LDL-C was(2.23±0.38)mmol/L,FPG were(5.43±0.64)mmol/L,and FINS was(9.35±1.96)mIU/L.LH was(6.49±1.23)IU/L,which was significantly higher than that of the control group(P<0.05).The HDL-C of patients in the PCOS group was(1.39±0.21)mmol/L,and the FSH was(5.68±1.14)IU/L,which were significantly lower than that of the control group(P<0.05).There was significant difference in the distribution of TT genotypes,CT genotypes,CC genotypes of T455C loci in case group compared with control group(P<0.05).There was significant difference in the distribution of AA genotypes,AC genotypes,CC genotypes of G201A loci in case group compared with control group(P<0.05).The multiple factor Logistic regression analysis showed that the polymorphism of the G201A,T455C locus of the IGF-1 gene and the high level of FINS in the serum were the risk factors for PCOS(P<0.05).Conclusion The polymorphism of IGF-1 gene is closely related to the occurrence of polycystic ovary syndrome,which canprovide experimental basis for the clinical treatment of patients.