麻黄素对小鼠肺组织抗氧化物酶和Bax,NF-kB表达的影响

Effects of ephedrine on antioxidant enzymes activities and Bax,NF-kB expression in mice lung

【目的】探讨麻黄素对肺组织抗氧化物酶活性和特异蛋白表达的影响.【方法】选48只小鼠随机分为麻黄素组和对照组,麻黄素组用递增剂量腹腔连续注射麻黄素溶液(2.0、4.0、6.0g/L)15d(每天两次,每次0.2mL),对照组注射等量生理盐水,分别于5、10、15d时,用比色法检测各组小鼠肺组织GSH-Px活性和MDA含量的变化,用免疫组织化学法检测肺组织Bax蛋白和NF-kB表达的变化.【结果】与对照组相比,麻黄素组小鼠肺组织GSH-Px活性降低,MDA含量升高,10、15d时差异显著或极显著(P<0.05或P<0.01),肺组织Bax蛋白和NF-kB阳性表达的平均光密度值增多,差异显著或极显著(P<0.05或P<0.01).【结论】麻黄素影响小鼠肺组织抗氧化物酶活性,能诱导肺组织细胞凋亡基因的表达,影响器官组织的结构和功能.

【Objective】To explore the effects of ephedrine on antioxidant enzymes activities and special protein expression of mice lung.【Method】48 mice were randomly divided into control group and ephedrine group.The ephedrine group mice intraperitoneal injected escalation doses(2.0 g/L,4.0 g/L,6.0 g/L)ephedrine(×0.2 mL),twice per day for 15 day,the control group mice injected with equal amount of saline.The changes of GSH-Px activity and MDA content in mice lung were analyzed by colorimetry.The expression changes of Bax protein and NF-κB were examined by immunohistochemical method.【Result】The activity of GSH-Px were significantly lower in the ephedrine group than those in the control group,while MDA content were significantly increased at the 10th and 15th treatment days(P<0.05 or P<0.01).The intensity of Bax protein and NF-κB positive expression were increased significantly in the lung(P<0.05 or P<0.01).【Conclusion】Ephedrine significantly affects the antioxidant enzyme activity of mice lung,may induces genic expression of cell apoptosis,then damages histological structure and function in mice lung.