屋久岛紫薇子叶愈伤组织诱导及植株再生技术初探

Callus induction and plant regeneration from the cotyledon of Lagerstroemia fauriei Koehne

以屋久岛紫薇子叶为外植体,通过添加不同种类和质量浓度的生长调节剂,对屋久岛紫薇进行组织培养试验,研究不同植物生长调节剂组合及质量浓度对屋久岛紫薇愈伤组织诱导、增殖及分化的影响,从而筛选出适宜屋久岛紫薇愈伤诱导、增殖和分化的培养条件。结果显示,屋久岛紫薇子叶外植体在组织培养过程中,适宜愈伤诱导培养基组合为1/2MS+1. 0 mg/L 6-BA+0. 5 mg/L 2,4-D,诱导率为46. 5%;愈伤组织增殖的适宜培养基为MS+1. 0 mg/L 6-BA+0. 1mg/L NAA,增殖系数最大,为3. 1;愈伤组织分化的最适培养基为WPM+1. 0 mg/L 6-BA+0. 5 mg/L IBA,分化率为18. 7%;移栽基质适宜为等容积的泥炭土+珍珠岩混合物,成活率为90%。

The present experiment was conducted to investigate regeneration from cotyledons of Lagerstroemia fauriei Koehne and establish its regeneration system,in order to provide a basis for its tissue culture and genetic transformation.Its cotyledons was used as explant and callus was induced by supplementation with different type and concentration of growth regulators.The results showed that the optimal culture medium for callus induction was1/2MS+1.0 mg/L 6-BA+0.5 mg/L 2,4-D,the highest frequency of callus induction was 46.5%.The optimal culture medium for multiplication was MS+1.0 mg/L 6-BA+0.1mg/L NAA,the multiplication coefficient was up to 3.1.The optimal culture medium for callus differentiation was WPM+1.0 mg/L 6-BA+0.5 mg/L IBA,the highest frequency of callus differentiation was 18.7%.The mixture of equal volume of peat+perlite was optimum culture substrate for transplantation.