miniSTR基因座及其检测系统在降解检材中的法医学应用

Application of mini STR Loci and Its Detection System for Degraded Materials in Forensic Medicine

目的建立16个miniSTR基因座的复合扩增体系,探讨其在法医学降解检材中的应用价值。方法采用六色荧光标记技术,对16个miniSTR基因座进行复合扩增体系的构建及法医学应用评估。结果开发出一个六色荧光标记的miniSTR扩增试剂盒,可同时对15个常染色体STR基因座、Amelogenin基因座和DYS391基因座进行分型检测,方法特异性好,分型结果稳定、准确,灵敏度达50 pg,实际案件中常见生物检材的检验结果良好。结论 16个miniSTR复合扩增体系对降解与微量检材的检测具有应用价值,灵敏度高,具有数据库兼容性,可以用于实际案件检验。

Objective To establish multiplex system of 16 miniSTR loci,and explore its application value for the degraded materials in forensic medicine.Methods The multiplex system of 16 miniSTR loci was established using a six-dye fluorescence labeling technology and its application value in forensic medicine was assessed.Results A six-dye fluorescence labeling miniSTR amplification kit was developed,which enabled 15 autosomal STR loci,Amelogenin locus and DYS391 to be typed simultaneously.This method showed good specificity and could provide stable and accurate typing results with a sensitivity of 50 pg.This system also provided a good test result for the normal biological sample of actual cases.Conclusion The multiplex system of 16 miniSTR loci has application value for degraded and trace materials with the advantages of high sensitivity and database compatibility,which can be used for forensic casework.